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Modeling Stroke in Mice: Focal Cortical Lesions by Photothrombosis
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Thrombus formation induced by laser in a mouse model.
Pablo Pérez1, Marcelo Alarcón2, Eduardo Fuentes2
1Department of Clinical Biochemistry and Immunohematology, Faculty of Health Sciences, Interdisciplinary Excellence Research Program on Healthy Aging (PIEI-ES), University of Talca, Talca, Maule 3460000, Chile.
Experimental and Therapeutic Medicine
|June 20, 2014
Summary
This study presents an accessible method for inducing and visualizing thrombosis in vivo using rose bengal and laser light in mice. The technique allows for real-time observation of thrombus formation, aiding thrombosis research.
Area of Science:
- Cardiovascular Research
- Hematology
- Medical Imaging
Background:
- Thrombosis pathophysiology research relies on animal models.
- Existing thrombosis induction techniques can be complex and costly.
- There is a need for more accessible in vivo models.
Purpose of the Study:
- To modify and simplify the technique for in vivo thrombosis induction.
- To establish a cost-effective and accessible method for studying thrombosis.
Main Methods:
- BALB/c mice were anesthetized and underwent abdominal surgery to isolate the mesentery.
- In vivo thrombosis was induced using rose bengal (25-50 mg/kg) and a 5 mW laser.
- Platelets were labeled with calcein acetyloxymethyl ester for real-time visualization.
Main Results:
- Thrombus formation was successfully induced within approximately 30 minutes.
- The induced thrombus occluded the mesenteric artery with a total area of 4,878.3 μm².
- Real-time visualization of thrombus development was achieved.
Conclusions:
- The modified technique provides a simple, low-cost, and accessible method for in vivo thrombosis studies.
- This approach is suitable for various research applications in thrombosis.
- The technique facilitates real-time observation of thrombus formation and progression.
