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Related Concept Videos

Next-generation Sequencing03:00

Next-generation Sequencing

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The first human genome sequencing project cost $2.7 billion and was declared complete in 2003, after 15 years of international cooperation and collaboration between several research teams and funding agencies. Today, with the advent of next-generation sequencing technologies, the cost and time of sequencing a human genome have dropped over 100 fold.
Next-Generation Sequencing Methods
Although all next-generation methods use different technologies, they all share a set of standard features....
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RNA-seq03:21

RNA-seq

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RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases. 
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while...
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Sanger Sequencing01:57

Sanger Sequencing

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DNA sequencing is a fundamental technique that is routinely used in the biological sciences. This method can be applied to a range of questions at different scales - from the sequencing of a cloned DNA fragment or the study of a mutation in a gene up to whole-genome sequencing. However, despite the widespread use of sequencing today, it was not until 1977 that Fredrick Sanger and his collaborators developed the chain-termination method to decode DNA sequences. It relies on the separation of a...
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Next Generation Sequencing for the Detection of Actionable Mutations in Solid and Liquid Tumors
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[Next generation sequencing -  application in clinical practice].

L Koubková, B Vojtěšek, R Vyzula

    Klinicka Onkologie : Casopis Ceske a Slovenske Onkologicke Spolecnosti
    |June 20, 2014
    PubMed
    Summary
    This summary is machine-generated.

    Next-generation sequencing (NGS) advances genomic research, enabling rapid, cost-effective human genome sequencing. NGS in cancer research offers deep insights into tumor complexity and identifies new mutations in hereditary cancer syndromes.

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    Area of Science:

    • Genomics
    • Cancer Research
    • Molecular Biology

    Context:

    • Genomic research has been revolutionized by the development of faster and more economical sequencing methods.
    • Next-generation sequencing (NGS) technologies have become instrumental in cancer research, providing comprehensive molecular characterization.
    • These advancements allow for deeper insights into tumor complexity, heterogeneity, and evolution.

    Purpose:

    • To review the technical characteristics of major next-generation sequencing platforms.
    • To provide an overview of the advantages and disadvantages of different NGS technologies.
    • To discuss the clinical perspective of applying NGS in genomic research and cancer studies.

    Summary:

    • NGS enables rapid and cost-effective determination of the complete human genome sequence.
    • Application of NGS in cancer research has led to the identification of new causal mutations in hereditary cancer syndromes.
    • Sequencing over 15 tumor types has revealed new cancer-driving mutations and genome structural rearrangements by comparing tumor cells to normal cells.

    Impact:

    • Accelerated pace of genomic research and discovery.
    • Enhanced understanding of cancer at a molecular level.
    • Potential for improved diagnostics and targeted therapies in hereditary cancer syndromes.