Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

IR Spectroscopy: Molecular Vibration Overview01:24

IR Spectroscopy: Molecular Vibration Overview

5.7K
When Infrared (IR) radiation passes through a covalently bonded molecule, the bonds transition from lower to higher vibrational levels. The fundamental vibrational motions that result in infrared absorption can be classified as stretching or bending vibrations.
Stretching vibrations are vibrational motions that occur along the bond line, changing the bond length or distance between two bonded atoms. They are further distinguished as symmetric or asymmetric. In symmetric stretching, the...
5.7K
IR Spectrum Peak Splitting: Symmetric vs Asymmetric Vibrations01:08

IR Spectrum Peak Splitting: Symmetric vs Asymmetric Vibrations

2.0K
Identical bonds within a polyatomic group can stretch symmetrically (in-phase) or asymmetrically (out-of-phase). Similar to hydrogen bonding, these vibrations also influence the shape of the IR peak. Generally, asymmetric stretching frequencies are higher than symmetric stretching frequencies. For example, primary amines exhibit two distinct IR peaks between 3300–3500 cm−1 corresponding to the symmetric and asymmetric N-H stretching, while secondary amines exhibit a single...
2.0K
IR Spectroscopy: Hooke's Law Approximation of Molecular Vibration01:16

IR Spectroscopy: Hooke's Law Approximation of Molecular Vibration

3.3K
A covalently bonded heteronuclear diatomic molecule can be modeled as two vibrating masses connected by a spring. The vibrational frequency of the bond can be expressed using an equation derived from Hooke's law, which describes how the force applied to stretch or compress a spring is proportional to the displacement of the spring. In this case, the atoms behave like masses, and the bond acts like a spring.
According to Hooke's law, the vibrational frequency is directly proportional to...
3.3K
Raman Spectroscopy: Overview01:20

Raman Spectroscopy: Overview

2.6K
The underlying principle of Raman spectroscopy is based on the interaction between light and matter, specifically molecules' inelastic scattering of photons. When a monochromatic beam of light, typically from a laser source, interacts with a sample, most scattered light has the same frequency as the incident light. This is known as Rayleigh scattering.
However, a small fraction of the scattered light exhibits a frequency shift due to the exchange of energy between the incident photons and...
2.6K
Raman Spectroscopy Instrumentation: Overview01:26

Raman Spectroscopy Instrumentation: Overview

1.8K
A conventional Raman spectrophotometer includes a laser source, a sample holding system, a wavelength selector, and a detector.
The monochromatic laser source, typically using visible or near-infrared radiation, generates a highly focused beam of light. This light interacts with the molecules of the sample, scattering some of the light. Liquid and gaseous samples are usually tested in ordinary glass capillaries, while solids can be analyzed as powders packed in capillaries or as potassium...
1.8K
¹H NMR: Interpreting Distorted and Overlapping Signals01:02

¹H NMR: Interpreting Distorted and Overlapping Signals

1.3K
Spin systems where the difference in chemical shifts of the coupled nuclei is greater than ten times J are called first-order spin systems. These nuclei are weakly coupled, and their chemical shifts and coupling constant can generally be estimated from the well-separated signals in the spectrum.
As Δν decreases and the signals move closer, the doublets appear increasingly distorted. The intensities of the inner lines increase at the cost of those of the outer lines as the signals are...
1.3K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Patterns in two-dimensional spectra can identify broken rovibrational selection rules.

The Journal of chemical physics·2025
Same author

Open-Hybrid Aortic Stent Placement for Recurrent Coarctation in Complex Single Ventricles.

Annals of thoracic surgery short reports·2025
Same author

Two-Dimensional Spectroscopy Isolates Infrared Rovibrational Patterns.

The journal of physical chemistry letters·2024
Same author

Multidimensional Pattern Recognition in High-Resolution 2D and 3D Spectra of Gas-Phase Molecules.

Accounts of chemical research·2023
Same author

High resolution two-dimensional infrared (HR-2DIR) spectroscopy of gas phase molecules.

The Journal of chemical physics·2022
Same author

Surgical approach for anomalous aortic origin of a coronary artery: A comparison of two techniques.

Journal of cardiac surgery·2021

Related Experiment Video

Updated: Apr 28, 2026

Author Spotlight: Unveiling the Potential of VSFG Microscopy in Studying Mesoscopically Heterogeneous Self-Assembled Structures
08:49

Author Spotlight: Unveiling the Potential of VSFG Microscopy in Studying Mesoscopically Heterogeneous Self-Assembled Structures

Published on: December 1, 2023

2.1K

Rotational and vibrational pattern interpretation for high-resolution coherent 3D spectroscopy.

Benjamin R Strangfeld1, Thresa A Wells, Peter C Chen

  • 1Chemistry Department, Spelman College , Atlanta, Georgia 30314, United States.

The Journal of Physical Chemistry. A
|June 20, 2014
PubMed
Summary

High-resolution coherent 3D spectroscopy offers enhanced resolution for complex molecular spectra. This technique aids in analyzing rotationally congested gas-phase electronic spectra, overcoming limitations of 2D methods.

More Related Videos

Direct Imaging of Laser-driven Ultrafast Molecular Rotation
10:52

Direct Imaging of Laser-driven Ultrafast Molecular Rotation

Published on: February 4, 2017

9.5K
A Multimodal Wide-Field Fourier-Transform Raman Microscope
06:48

A Multimodal Wide-Field Fourier-Transform Raman Microscope

Published on: December 30, 2025

858

Related Experiment Videos

Last Updated: Apr 28, 2026

Author Spotlight: Unveiling the Potential of VSFG Microscopy in Studying Mesoscopically Heterogeneous Self-Assembled Structures
08:49

Author Spotlight: Unveiling the Potential of VSFG Microscopy in Studying Mesoscopically Heterogeneous Self-Assembled Structures

Published on: December 1, 2023

2.1K
Direct Imaging of Laser-driven Ultrafast Molecular Rotation
10:52

Direct Imaging of Laser-driven Ultrafast Molecular Rotation

Published on: February 4, 2017

9.5K
A Multimodal Wide-Field Fourier-Transform Raman Microscope
06:48

A Multimodal Wide-Field Fourier-Transform Raman Microscope

Published on: December 30, 2025

858

Area of Science:

  • Physical Chemistry
  • Molecular Spectroscopy
  • Quantum Optics

Background:

  • Conventional methods struggle with rotationally resolved electronic spectra of gas-phase molecules due to spectral congestion.
  • High-resolution coherent 2D spectroscopy improves pattern recognition but can still suffer from peak overlap in complex spectra.

Purpose of the Study:

  • To introduce and guide the application of high-resolution coherent 3D spectroscopy for analyzing severely congested molecular spectra.
  • To provide essential information for planning, executing, and interpreting 3D spectroscopic experiments.

Main Methods:

  • Development and demonstration of a 3D extension to high-resolution coherent multidimensional spectroscopy.
  • Utilizing enhanced spectral resolution and selectivity offered by the 3D technique.

Main Results:

  • The 3D technique demonstrates superior resolution and selectivity compared to 2D methods, effectively addressing severe spectral congestion.
  • Identified patterns in spectra that were previously obscured by rotational congestion.

Conclusions:

  • High-resolution coherent 3D spectroscopy is a powerful advancement for analyzing complex gas-phase molecular electronic spectra.
  • The 3D approach significantly enhances the ability to resolve and interpret spectral data where 2D methods fall short.