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Updated: Apr 28, 2026

Assessment of Viability of Human Fat Injection into Nude Mice with Micro-Computed Tomography

Assessment of Viability of Human Fat Injection into Nude Mice with Micro-Computed Tomography

Published on: January 7, 2015

Comparative analysis of processing methods in fat grafting.

Harry M Salinas¹, G Felix Broelsch, Justin R Fernandes

¹Boston, Mass. From the Division of Plastic and Reconstructive Surgery, Massachusetts General Hospital.

Plastic and Reconstructive Surgery

|June 20, 2014

Summary

This summary is machine-generated.

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Centrifugation effectively concentrates fat for improved graft retention. This method is as effective as the mesh/gauze technique for concentrating fat and isolating stem cells.

Area of Science:

Biomedical Engineering
Regenerative Medicine
Tissue Engineering

Background:

Centrifugation is a common method for processing lipoaspirate, but its mechanism of action is not fully understood.
Hypotheses regarding its benefits include fat concentration, reduced inflammation, and concentration of adipose-derived stem cells.
This study investigates the role of centrifugation in fat grafting and compares it to the mesh/gauze technique.

Purpose of the Study:

To determine the concentration efficacy of centrifugation at various speeds.
To compare the concentration efficacy of centrifugation with the mesh/gauze technique.
To evaluate the impact of different lipoaspirate components on fat graft retention in a mouse model.

Main Methods:

Quantified lipoaspirate components after centrifugation at increasing speeds.

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Quantified concentration efficacy of the mesh/gauze technique.

Compared adipose-derived stem cell counts between centrifugation and mesh/gauze methods.

Assessed fat graft retention in a mouse model using fat mixed with various components.

Main Results:

Centrifugation at 1200 g achieved 91% fat concentration, comparable to 90% with mesh/gauze.
Adipose-derived stem cell counts were similar between centrifugation and mesh/gauze groups (p = 0.86).
Fat-only grafts showed better retention than those mixed with tumescence or cell pellets; oil did not affect grafts.

Conclusions:

Centrifugation enhances fat graft retention by concentrating the adipocyte fraction.
The mesh/gauze technique demonstrates equivalent fat concentration efficacy and in vivo outcomes compared to centrifugation at 1200 g.
Optimal fat grafting involves concentrating adipocytes and avoiding additives like tumescence.