Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Protein Networks

Protein Networks

An organism can have thousands of different proteins, and these proteins must cooperate to ensure the health of an organism. Proteins bind to other proteins and form complexes to carry out their functions. Many proteins interact with multiple other proteins creating a complex network of protein interactions.
These interactions can be represented through maps depicting protein-protein interaction networks, represented as nodes and edges. Nodes are circles that are representative of a protein,...

Proteomics

Proteomics

A proteome is the entire set of proteins that a cell type produces. We can study proteomes using the knowledge of genomes because genes code for mRNAs, and the mRNAs encode proteins. Although mRNA analysis is a step in the right direction, not all mRNAs are translated into proteins.
Proteomics is the study of proteomes' function. It involves the large-scale systematic study of the proteome to denote the protein complement expressed by a genome. Scientist Mark Wilkins coined the term...

Protein-protein Interfaces

Protein-protein Interfaces

Many proteins form complexes to carry out their functions, making protein-protein interactions (PPIs) essential for an organism's survival. Most PPIs are stabilized by numerous weak noncovalent chemical forces. The physical shape of the interfaces determines the way two proteins interact. Many globular proteins have closely-matching shapes on their surfaces, which form a large number of weak bonds. Additionally, many PPIs occur between two helices or between a surface cleft and a...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

A chemoproteomic biotechnological toolkit for resolving xylanase specificity in decorated xylan.

Nature communications·2026

Same author

Oseltamivir aziridines are potent influenza neuraminidase inhibitors and imaging agents.

Proceedings of the National Academy of Sciences of the United States of America·2026

Same author

Fully protected pyrophosphates <i>via</i> phosphorobromidates for the synthesis of biopolymers.

Chemical science·2026

Same author

Catalyzing Carbohydrate Cleavage: Glycosidases and Their Mechanisms.

Chemical reviews·2026

Same author

ChIP happens: from biochemical origins to the modern omics toolbox for understanding steroid hormone receptors.

The Biochemical journal·2026

Same author

Xylosyltransferase engineering to manipulate proteoglycans in mammalian cells.

Nature chemical biology·2026

Same journal

Neutral Amphiphiles Boost Transfection Efficiency and Reduce Inflammation in Polymer Micelle-Mediated mRNA Delivery.

Bioconjugate chemistry·2026

Same journal

Surfactant-Mediated Buchwald-Hartwig Coupling of Aliphatic Amines for the Synthesis of DNA-Encoded Libraries.

Bioconjugate chemistry·2026

Same journal

Artificial Intelligence for Discovery in Life Sciences.

Bioconjugate chemistry·2026

Same journal

Iron Single Atom Nanozyme-Mediated GPX4 Inhibitor Delivery for Self-Enhanced Ferroptosis.

Bioconjugate chemistry·2026

Same journal

SpyCatcher-Engineered Ferritin Nanocages Enable Dual-Receptor Targeting for Enhanced Glioma Therapy.

Bioconjugate chemistry·2026

Same journal

One-Pot Synthesis of Functionalized Coumarin Fluorophores Enables Rapid Access to Live-Cell Bioorthogonal Labeling and Microenvironmental Sensing Agents.

Bioconjugate chemistry·2026

See all related articles

Search research articles

Related Experiment Video

Updated: Feb 18, 2026

Split-BioID — Proteomic Analysis of Context-specific Protein Complexes in Their Native Cellular Environment

Split-BioID — Proteomic Analysis of Context-specific Protein Complexes in Their Native Cellular Environment

Published on: April 20, 2018

Current developments in activity-based protein profiling.

Lianne I Willems¹, Herman S Overkleeft, Sander I van Kasteren

¹Leiden University , Leiden Institute of Chemistry, Gorlaeus Laboratories, Einsteinweg 55, 2333 CC Leiden, The Netherlands.

Bioconjugate Chemistry

|June 20, 2014

Summary

This summary is machine-generated.

Activity-based protein profiling (ABPP) uses chemical probes to visualize active enzymes in proteomes. This technique aids in understanding enzyme function, disease states, inhibitor discovery, and natural product target identification.

More Related Videos

Resolving Affinity Purified Protein Complexes by Blue Native PAGE and Protein Correlation Profiling

Resolving Affinity Purified Protein Complexes by Blue Native PAGE and Protein Correlation Profiling

Published on: April 1, 2017

Probing High-density Functional Protein Microarrays to Detect Protein-protein Interactions

Probing High-density Functional Protein Microarrays to Detect Protein-protein Interactions

Published on: August 2, 2015

Related Experiment Videos

Last Updated: Feb 18, 2026

Split-BioID — Proteomic Analysis of Context-specific Protein Complexes in Their Native Cellular Environment

Split-BioID — Proteomic Analysis of Context-specific Protein Complexes in Their Native Cellular Environment

Published on: April 20, 2018

Resolving Affinity Purified Protein Complexes by Blue Native PAGE and Protein Correlation Profiling

Resolving Affinity Purified Protein Complexes by Blue Native PAGE and Protein Correlation Profiling

Published on: April 1, 2017

Probing High-density Functional Protein Microarrays to Detect Protein-protein Interactions

Probing High-density Functional Protein Microarrays to Detect Protein-protein Interactions

Published on: August 2, 2015

Area of Science:

Biochemistry
Chemical Biology
Proteomics

Background:

Activity-based protein profiling (ABPP) is a key strategy for studying enzyme activity within complex biological systems.
It utilizes specialized chemical probes, known as activity-based probes (ABPs), to selectively target and label active enzymes.

Purpose of the Study:

To provide an overview of recent advancements in the field of activity-based protein profiling.
To highlight the versatility of ABPP in various biological and chemical research applications.

Main Methods:

ABPP employs mechanism-based reactive probes that covalently bind to the active site of target enzymes.
This selective labeling allows for the visualization of only catalytically active enzyme forms.

Main Results:

ABPP enables the monitoring of specific enzymatic activities in real-time.
It facilitates the identification of novel protein functions and the characterization of enzyme roles in disease.
ABPP is instrumental in discovering and evaluating enzyme inhibitors and identifying targets of natural products.

Conclusions:

Activity-based protein profiling is a powerful and versatile tool in biochemical and chemical biology research.
Recent developments continue to expand the applications of ABPP in understanding complex biological processes and drug discovery.