[Immunosensor for rapid detection of 1,3-dinitrobenzene].

Area of Science:

• Analytical Chemistry
• Biosensing Technology

Background:

• 1,3-dinitrobenzene is a toxic environmental pollutant.
• Rapid and accurate detection methods are crucial for water quality monitoring.

Purpose of the Study:

• To develop a novel immunosensor for the rapid detection of 1,3-dinitrobenzene.
• To evaluate the performance and reliability of the developed immunosensor.

Main Methods:

• Development of an evanescent wave all-fiber biosensing platform.
• Synthesis and immobilization of hapten-carrier conjugates (NB-OVA).
• Characterization of probe robustness, regeneration, and assay performance.

Main Results:

• Achieved a detection limit of 0.054 mg/L for 1,3-dinitrobenzene.
• Detection cycle time was less than 10 minutes.
• Demonstrated high precision (80-120% recovery) and accuracy in real water samples with negligible matrix effects.
• The probe supported over 100 assay cycles without significant reactivity loss.

Conclusions:

• The developed immunosensor provides a rapid, sensitive, and accurate method for detecting 1,3-dinitrobenzene.
• The biosensing platform exhibits excellent robustness and reusability, suitable for practical water analysis.
• This technology holds significant potential for environmental monitoring and water safety applications.