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Related Concept Videos

Chromatographic Methods: Terminology01:18

Chromatographic Methods: Terminology

3.9K
Chromatography is an analytical technique widely used in fields such as chemistry, biology, environmental science, and pharmaceuticals to separate the components of a mixture and identify substances between them. The process of chromatography is based on the interactions between two distinct phases: the stationary phase and the mobile phase. The stationary phase is fixed in place by a supporting material, while the mobile phase moves over it, carrying the solutes. As the mobile phase travels,...
3.9K
Chromatographic Resolution01:15

Chromatographic Resolution

2.4K
In chromatography, a solute moves through a chromatographic column and tends to spread, forming a Gaussian-shaped band. The longer the solute spends in the column, the broader the band becomes. The broadening can lead to overlaps within the column, affecting separation effectiveness.
The effectiveness of separation can be evaluated by determining the level of separation between two neighboring peaks in a chromatogram, which represents the individual components of a sample.
In chromatography,...
2.4K
Optimizing Chromatographic Separations01:15

Optimizing Chromatographic Separations

1.3K
Optimizing chromatographic separations is crucial for obtaining clean separations in a minimum amount of time. Optimization is required for several factors, including kinetic effects related to band broadening, plate height, capacity factor, and separation factor.
Band broadening refers to spreading solute bands as they travel through the column. This broadening can impact resolution. Plate height (H) represents the length required for one theoretical plate. A lower plate height corresponds to...
1.3K
Principles Of Column Chromatography01:13

Principles Of Column Chromatography

7.3K
The chromatography technique was first invented in 1901 by Michael S. Tswett, a Russian botanist, to separate plant pigments using organic solvents. Further, in 1941, Archer John Porter Martin and R. L. M. Synge modified the technique by packing silica gel into a column. A mixture of amino acids was then separated on the packed column using chloroform and water mixture as the mobile phase. This was the first report on column chromatography. At present, column chromatography is a widely used...
7.3K
High-Performance Liquid Chromatography: Introduction01:11

High-Performance Liquid Chromatography: Introduction

3.0K
High-performance liquid chromatography(HPLC), formerly referred to as High-pressure liquid chromatography, is a powerful technique used to separate, identify, and quantify components in complex mixtures. The term "high pressure" refers to using high pressure to push the liquid mobile phase through the tightly packed columns.
In HPLC, two phases play a critical role in the separation process:
3.0K
Chromatographic Methods: Classification01:12

Chromatographic Methods: Classification

3.5K
Chromatographic techniques are classified in three ways: the classification is based on the physical state of the stationary and mobile phases, how the mobile phase and the stationary phase contact each other, or through the chemical or physical processes that isolate the components of the sample. Typically, the mobile phase is either a liquid or gas, while the stationary phase is either a solid or a liquid layer applied to a solid surface.
Chromatographic techniques are typically named by...
3.5K

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Updated: Apr 27, 2026

Chromatographic Fingerprinting by Template Matching for Data Collected by Comprehensive Two-Dimensional Gas Chromatography
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Ask the experts: chromatographic baselines.

Graeme Smith, Christopher A James, Rebecca Scott

    Bioanalysis
    |June 21, 2014
    PubMed
    Summary
    This summary is machine-generated.

    Leading researchers discuss challenges in automated chromatographic baseline assignment and the necessity of peak reintegration in bioanalytical laboratories. Their insights aim to foster industry consensus on these critical analytical procedures.

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    Liquid Chromatography Coupled to Refractive Index or Mass Spectrometric Detection for Metabolite Profiling in Lysate-based Cell-free Systems
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    Large Scale Non-targeted Metabolomic Profiling of Serum by Ultra Performance Liquid Chromatography-Mass Spectrometry UPLC-MS
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    Area of Science:

    • Analytical Chemistry
    • Bioanalysis
    • Chromatography

    Background:

    • The accurate assignment of chromatographic baselines is fundamental in bioanalytical method validation.
    • Automated baseline assignment presents significant challenges in complex biological matrices.
    • The necessity and implications of peak reintegration require careful consideration.

    Discussion:

    • Expert opinions were gathered on the complexities of automated baseline assignment.
    • The criteria and timing for performing peak reintegration were debated.
    • Regulatory expectations and the need for consistent terminology were highlighted.

    Key Insights:

    • Developing an industry consensus on chromatographic baseline assignment is crucial.
    • Addressing the challenges of automated methods requires expert input.
    • Standardizing terminology will improve clarity and reproducibility.

    Outlook:

    • Further dialogue is needed to establish industry-wide best practices.
    • Future research may focus on refining automated baseline algorithms.
    • Harmonization of guidelines will enhance the reliability of bioanalytical data.