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The effect of caffeine on MDMA-induced hydroxyl radical production in the mouse striatum
Anna Maria Górska1, Karolina Noworyta-Sokołowska1, Krystyna Gołembiowska1
1Institute of Pharmacology, Polish Academy of Sciences, Kraków, Poland.
Background:
The psychostimulant 3,4-methylenedioxymethamphetamine (MDMA) with a strong addictive potential is widely used as a recreational drug. Neurotoxicity of MDMA is related with the generation of highly reactive free radicals.
Methods:
MDMA was given in doses of 20 and 40mg/kg ip alone or in combination with caffeine (CAF) 10mg/kg ip. Extracellular concentration of hydroxyl radical was measured using microdialysis in freely moving mice and was assayed by HPLC with electrochemical detection.
Results:
MDMA dose-dependently increased production of hydroxyl radical in the mouse striatum and its effect was reversed by caffeine.
Conclusions:
The data show that caffeine may have neuroprotective properties as it decreased oxidative stress induced by MDMA.
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