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Updated: Apr 27, 2026

Ion Exchange Chromatography IEX Coupled to Multi-angle Light Scattering MALS for Protein Separation and Characterization
Published on: April 5, 2019
Liquid chromatographic enantiomer separation with special focus on zwitterionic chiral ion-exchangers
1Institute of Pharmaceutical Sciences, University of Tübingen, Auf der Morgenstelle 8, 72076, Tübingen, Germany, michael.laemmerhofer@uni-tuebingen.de.
Abstract:
This article provides a condensed introduction to principles of chiral separation, gives a historic overview of the genesis of the most important concepts regarding chiral stationary phase (CSPs), and summarizes the state of the art in a concise manner. Some recent developments in the field of polysaccharide CSPs are outlined. Finally, the article focusses on the new concept of zwitterionic chiral stationary phases and their application profile and peculiarities. Some other trends in column technology, including sub-2 μm and core-shell CSP particles and the emerging field of (UP)SFC, are briefly discussed.
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