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Expression of Recombinant Cellulase Cel5A from Trichoderma reesei in Tobacco Plants

Expression of Recombinant Cellulase Cel5A from Trichoderma reesei in Tobacco Plants

Published on: June 13, 2014

Erythritol production on wheat straw using Trichoderma reesei.

Birgit Jovanović¹, Robert L Mach¹, Astrid R Mach-Aigner¹

¹Department for Biotechnology and Microbiology, Institute of Chemical Engineering, Vienna University of Technology, Gumpendorfer Str. 1a, A-1060 Wien, Austria.

|June 21, 2014

Summary

Overexpressing the err1 gene in Trichoderma reesei enhanced erythritol production, particularly in the Rut-C30 strain using lignocellulosic substrates like wheat straw.

Keywords:

Erythritol Erythrose reductase Trichoderma reesei; Wheat straw; Lignocellulose

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Area of Science:

Biotechnology
Industrial Microbiology
Metabolic Engineering

Background:

Trichoderma reesei is a fungus known for its cellulase production capabilities.
Erythritol is a sugar alcohol with various industrial applications.
Enhancing erythritol production in T. reesei could offer a sustainable bioprocess.

Purpose of the Study:

To investigate the feasibility of erythritol production in Trichoderma reesei.
To evaluate the overexpression of the err1 gene for enhanced erythritol yield.
To assess production using both D-xylose and pretreated lignocellulosic substrates.

Main Methods:

Overexpression of the err1 gene in wild-type and Rut-C30 T. reesei strains using constitutive (pki) and inducible (bxl1) promoters.
Characterization of err1 transcript formation on D-xylose and xylan.
Erythritol production experiments in shake flasks and bioreactors.
Utilization of alkaline organosolve pretreated wheat straw as a lignocellulosic substrate.

Main Results:

Shake flask experiments showed increased erythritol formation in both wild-type and Rut-C30 strains overexpressing err1 compared to parental strains.
Bioreactor cultivation on wheat straw did not enhance erythritol production in the wild-type overexpression strain.
Overexpression of err1 in the Rut-C30 strain significantly increased erythritol formation on wheat straw.

Conclusions:

The err1 gene is a viable target for enhancing erythritol production in T. reesei.
The Rut-C30 strain, a cellulase hyperproducer, shows superior performance for erythritol synthesis when err1 is overexpressed, especially on lignocellulosic materials.
This study demonstrates the potential of engineered T. reesei for sustainable erythritol production from agricultural waste.