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DNA Isolation

DNA Isolation

DNA from cells is required for many biotechnology and research applications, such as molecular cloning. To remove and purify DNA from cells, researchers use various methods of DNA extraction. While the specifics of different protocols may vary, some general concepts underlie the process of DNA extraction.

DNA Isolation

DNA Isolation

DNA isolation protocols can be fast and straightforward or complex and time-consuming depending on the type and quality of DNA required for further processing. For example, plasmid DNA extraction is a bit more complicated than genomic DNA extraction because of the need for an appropriate lysis method to separate plasmid DNA from gDNA during isolation. However, for specific applications, such as long-range DNA sequencing that require a good yield of high- quality DNA samples, we need to follow...

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Related Experiment Video

Updated: Apr 27, 2026

Single-step Purification of Macromolecular Complexes Using RNA Attached to Biotin and a Photo-cleavable Linker

Single-step Purification of Macromolecular Complexes Using RNA Attached to Biotin and a Photo-cleavable Linker

Published on: January 3, 2019

Ribonucleic acid purification.

R Martins¹, J A Queiroz¹, F Sousa¹

¹CICS-UBI - Health Sciences Research Centre, University of Beira Interior, Av. Infante D. Henrique, 6200-506 Covilhã, Portugal.

Journal of Chromatography. A

|June 22, 2014

Summary

This summary is machine-generated.

New chromatographic methods using amino acids like histidine and arginine can effectively purify RNA. These advancements are crucial for improving RNA quality for both research and therapeutic applications.

Keywords:

Affinity chromatography Amino acids Isolation methods Purification RNA

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Published on: May 25, 2019

Area of Science:

Biochemistry
Molecular Biology
Biotechnology

Background:

Ribonucleic acid (RNA) research is vital for biological discoveries and therapeutic advancements.
RNA's susceptibility to degradation necessitates pure and intact molecules for research and clinical use.
Existing RNA isolation and purification methods have limitations, driving the need for improved techniques.

Purpose of the Study:

To discuss the significance and advancements in RNA isolation and purification techniques.
To explore the application of RNA in research and as biotherapeutic tools.
To highlight recent progress in chromatographic methods for RNA purification.

Main Methods:

Review of current RNA isolation and purification procedures.
Focus on chromatographic bioseparation, particularly affinity chromatography.
Investigation of biorecognition between RNA and amino acid ligands (histidine, arginine).

Main Results:

Affinity chromatography shows high specificity for RNA purification.
Histidine and arginine ligands effectively isolate various RNA species.
Demonstrated multipurpose applicability of these methods in molecular biology and therapeutics.

Conclusions:

Advancements in chromatography offer promising solutions for RNA purification challenges.
Amino acid-based affinity chromatography can enhance RNA quality for regulatory demands.
These methods support the growing use of RNA in research and biopharmaceutical development.