Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Development of a Brain-Penetrant Nurr1 Agonist Tool.

ChemMedChem·2026
Same author

DHODH Inhibitors Based on the Vidofludimus Scaffold Containing Carboxylic Acid Bioisosteres Exert a Superior Broad-Spectrum Antiviral Activity.

Journal of medicinal chemistry·2026
Same author

Clinical development of tacrolimus-resistant regulatory T cells to enable simultaneous immunosuppression and immune regulation.

Molecular therapy. Advances·2026
Same author

KDM6 Enzymes are the Mechanistic Targets of Mutant IDH that Dictate Replication Stress Sensitivity.

bioRxiv : the preprint server for biology·2026
Same author

An Open-label, Phase 1 Dose-finding Study of Single-agent IMU-935, a Novel RORγ/RORγt Inverse Agonist, in Patients with Progressive Metastatic Castration-resistant Prostate Cancer.

European urology oncology·2026
Same author

Personalized signaling pathway analysis of gastrointestinal tumors for patient stratification and drug target evaluation using clinically derived core biopsies.

NPJ precision oncology·2026

Related Experiment Video

Updated: Apr 27, 2026

2 in 1: One-step Affinity Purification for the Parallel Analysis of Protein-Protein and Protein-Metabolite Complexes
08:23

2 in 1: One-step Affinity Purification for the Parallel Analysis of Protein-Protein and Protein-Metabolite Complexes

Published on: August 6, 2018

12.7K

RPPApipe: a pipeline for the analysis of reverse-phase protein array data.

Johannes Eichner1, Yvonne Heubach2, Manuel Ruff1

  • 1Center for Bioinformatics Tuebingen (ZBIT), University of Tuebingen, Tübingen 72076, Germany.

Bio Systems
|June 22, 2014
PubMed
Summary

RPPApipe is a new web-based data analysis pipeline designed for reverse-phase protein arrays (RPPA). It offers comprehensive tools for RPPA data preprocessing, statistical analysis, and pathway analysis.

Keywords:
ClusteringPathway analysisReverse-phase protein arraysStatistical analysisVisualizationWeb application

More Related Videos

The Use of Reverse Phase Protein Arrays RPPA to Explore Protein Expression Variation within Individual Renal Cell Cancers
12:22

The Use of Reverse Phase Protein Arrays RPPA to Explore Protein Expression Variation within Individual Renal Cell Cancers

Published on: January 22, 2013

33.1K
Bidirectional Retroviral Integration Site PCR Methodology and Quantitative Data Analysis Workflow
12:53

Bidirectional Retroviral Integration Site PCR Methodology and Quantitative Data Analysis Workflow

Published on: June 14, 2017

10.2K

Related Experiment Videos

Last Updated: Apr 27, 2026

2 in 1: One-step Affinity Purification for the Parallel Analysis of Protein-Protein and Protein-Metabolite Complexes
08:23

2 in 1: One-step Affinity Purification for the Parallel Analysis of Protein-Protein and Protein-Metabolite Complexes

Published on: August 6, 2018

12.7K
The Use of Reverse Phase Protein Arrays RPPA to Explore Protein Expression Variation within Individual Renal Cell Cancers
12:22

The Use of Reverse Phase Protein Arrays RPPA to Explore Protein Expression Variation within Individual Renal Cell Cancers

Published on: January 22, 2013

33.1K
Bidirectional Retroviral Integration Site PCR Methodology and Quantitative Data Analysis Workflow
12:53

Bidirectional Retroviral Integration Site PCR Methodology and Quantitative Data Analysis Workflow

Published on: June 14, 2017

10.2K

Area of Science:

  • Bioinformatics
  • Computational Biology
  • Genomics

Background:

  • Existing web-based data analysis pipelines offer limited support for reverse-phase protein arrays (RPPA).
  • Current tools often fail to account for the unique properties of RPPA datasets.
  • This necessitates specialized software for effective RPPA data analysis.

Purpose of the Study:

  • To develop RPPApipe, a web-based data analysis pipeline specifically tailored for RPPA data.
  • To provide a comprehensive suite of tools for RPPA data preprocessing, statistical analysis, clustering, and pathway analysis.
  • To enhance the analysis capabilities for RPPA datasets in biological research.

Main Methods:

  • RPPApipe was developed using R/Bioconductor.
  • The software is integrated into the web-based ZBIT Bioinformatics Toolbox, a customized Galaxy platform instance.
  • The pipeline includes tools for data preprocessing, statistical analysis, clustering, and pathway analysis.

Main Results:

  • RPPApipe provides tailored support for RPPA data characteristics.
  • The pipeline integrates various analytical tools for comprehensive RPPA data exploration.
  • The software is implemented within a user-friendly web-based platform.

Conclusions:

  • RPPApipe addresses the limitations of existing tools for RPPA data analysis.
  • The developed pipeline offers a specialized and comprehensive solution for RPPA researchers.
  • RPPApipe enhances the ability to perform in-depth analysis of RPPA datasets.