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Related Experiment Videos

Transmissible mupirocin resistance in Staphylococcus aureus.

M Rahman1, W C Noble, B Cookson

  • 1Department of Microbiology, Institute of Dermatology, United Medical School, London.

Epidemiology and Infection
|April 1, 1989
PubMed
Summary

High-level mupirocin resistance in Staphylococcus aureus strains spread easily between bacteria and on mouse skin. This resistance was linked to chromosomal DNA, not plasmids, indicating a novel genetic mechanism for antibiotic resistance.

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Area of Science:

  • Microbiology
  • Molecular Biology
  • Genetics

Background:

  • Antibiotic resistance is a growing global health concern.
  • Staphylococcus aureus is a common pathogen, and resistance to antibiotics like mupirocin complicates treatment.
  • Understanding the mechanisms of resistance spread is crucial for infection control.

Purpose of the Study:

  • To describe the spread of high-level mupirocin resistance in Staphylococcus aureus.
  • To investigate the genetic basis of this resistance.
  • To determine the transferability of the resistance.

Main Methods:

  • Filter mating experiments were used to assess resistance transfer between S. aureus strains.
  • In vivo studies on mouse skin were conducted to evaluate resistance spread.

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  • Agarose gel electrophoresis and caesium chloride gradient centrifugation were employed to detect plasmids.
  • Chromosomal DNA from resistant and sensitive strains was analyzed using DNA cleavage techniques.

    • Main Results:

    • Two strains of Staphylococcus aureus exhibiting high-level mupirocin resistance were identified.
    • The resistance was readily transferable to other S. aureus strains via filter mating.
    • Resistance transfer was also demonstrated on the skin of mice.
    • No plasmid DNA correlated with the resistance was detected using standard methods.
    • Analysis of chromosomal DNA revealed unique DNA bands in resistant strains compared to sensitive ones.

    Conclusions:

    • High-level mupirocin resistance in these S. aureus strains is transferable.
    • The genetic determinant for resistance appears to be integrated into the bacterial chromosome.
    • This finding suggests a chromosomal mechanism for the spread of mupirocin resistance in S. aureus.