Related Experiment Video
Updated: Apr 27, 2026

Measuring the 50% Haemolytic Complement CH50 Activity of Serum
Published on: March 29, 2010
A universal method for measuring functional activity of complement in humans, laboratory, domestic, and agricultural
O N Kuleshina1, L V Kozlov, E G Cheremnykh
1G. N. Gabrichevsky Moscow Research Institute of Epidemiology and Microbiology, Federal Service on Surveillance for Consumer Rights Protection and Human Wellbeing, Moscow, Russia.
Abstract:
A new universal method for measuring activity of the serum complement system in humans, laboratory, domestic, agricultural animals, birds and amphibians is based on automated evaluation of the mortality of ciliate Tetrahymena pyriformis under the effect of the complement system. In contrast to the hemolytic method, measured activity of the complement shows no erroneously high results caused by reactive lysis in febrile patients. The method can be used for studies of the complement system in humans and animals without species-specific adaptation.
Related Concept Videos
Complementation Tests
Organisms heterozygous for different mutations are crossed pairwise in all combinations. If present on different genes, the mutations can complement each other by providing the missing...
Complement System
Toxicity Testing in Animals

