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Genome-Wide Analysis of DNA Methylation in Gastrointestinal Cancer
Published on: September 18, 2020
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Different methylation profiles between intestinal and diffuse sporadic gastric carcinogenesis
Misuk Yang1, Hyun-Soo Kim1, Mee Yon Cho2
1Division of Gastroenterology, Department of Internal Medicine, Yonsei University Wonju College of Medicine, Wonju, Republic of Korea.
Clinics and Research in Hepatology and Gastroenterology
|June 24, 2014
Summary
Gastric cancer methylation patterns differ between intestinal and diffuse types. Signet ring cell carcinoma shows distinct epigenetic profiles from poorly differentiated carcinoma, indicating unique gastric cancer development pathways.
Area of Science:
- Oncology
- Epigenetics
- Molecular Biology
Background:
- Gastric cancer (GC) is classified into intestinal and diffuse types.
- Diffuse GC includes poorly differentiated carcinoma (PDC) and signet ring cell carcinoma (SRCC).
- SRCC may represent an early stage of diffuse GC that can progress to PDC.
Purpose of the Study:
- To investigate epigenetic methylation changes in gastric cancer.
- To correlate methylation patterns with histologic differentiation status in GC.
- To identify molecular differences between intestinal and diffuse type GCs, and between SRCC and PDC.
Main Methods:
- Analyzed DNA methylation status of 6 genes (GDNF, RORA, MINT25, KLF7, CDH1, LINE-1) using pyrosequencing.
- Included 149 paraffin-embedded and 115 fresh biopsy tissue samples.
- Compared methylation profiles across normal tissues, gastric dysplasia (GD), differentiated cancers (DCs), PDCs, and SRCCs.
Main Results:
- LINE-1 was hypomethylated in GCs versus normal and GD tissues.
- GDNF, RORA, and MINT25 were hypermethylated in intestinal GCs compared to diffuse GCs; CDH1 showed the reverse.
- SRCCs exhibited lower methylation of GDNF, RORA, MINT25, KLF7, and higher methylation of CDH1 compared to PDCs.
Conclusions:
- Intestinal and diffuse type GCs display distinct epigenetic methylation profiles.
- SRCC and PDC have different methylation profiles, suggesting unique molecular pathways in gastric carcinogenesis.
- Epigenetic alterations are key in understanding GC differentiation and progression.
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