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Related Concept Videos

In-situ Hybridization02:31

In-situ Hybridization

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In situ hybridization (ISH) is a technique used to detect and localize specific DNA or RNA molecules in cells, tissue, or tissue sections using a labeled probe. The technique was first used in 1969 for the investigation of nucleic acids. It is currently an essential tool in scientific research and clinical settings, especially for diagnostic purposes.
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Agarose gel electrophoresis is very useful in separating DNA fragments by size. Running a DNA ladder containing fragments of the known length alongside the sample helps determine the approximate length of the sample DNA fragments. However, additional steps are needed to verify the sequence identity of the sample DNA fragments.
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Microbial biosensors are analytical devices that utilize living microbes to detect specific substances through measurable signals. These devices consist of two main components: biosensing organisms and signal-transducing elements. Biosensing organisms, such as Escherichia coli or Saccharomyces cerevisiae, are typically housed in multiwell plates connected to transducers, enabling rapid, real-time detection of target analytes.Signal Generation MechanismWhen a target analyte—such as...
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Related Experiment Video

Updated: Apr 27, 2026

A Microfluidic-based Electrochemical Biochip for Label-free DNA Hybridization Analysis
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In-situ DNA hybridization detection with a reflective microfiber grating biosensor.

Dandan Sun1, Tuan Guo1, Yang Ran1

  • 1Institute of Photonics Technology, Jinan University, Guangzhou 510632, China.

Biosensors & Bioelectronics
|June 24, 2014
PubMed
Summary

This study introduces a novel fiber-optic biosensor using a microfiber Bragg grating for label-free DNA hybridization detection. The sensor achieves accurate measurements by compensating for temperature changes, detecting DNA down to 0.5 µM.

Keywords:
DNA hybridization detectionMicrofiber gratingOptical biosensorOptical fiber

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Area of Science:

  • Biomedical Engineering
  • Materials Science
  • Optoelectronics

Background:

  • Label-free biosensing is crucial for real-time biological detection.
  • Fiber-optic sensors offer advantages in sensitivity and remote monitoring.
  • Microfiber Bragg gratings (mFBGs) provide a robust platform for sensing applications.

Purpose of the Study:

  • To develop and demonstrate a label-free fiber-optic biosensor for in-situ DNA hybridization detection.
  • To achieve temperature-compensated refractive index (RI) measurements using a mFBG.
  • To evaluate the specificity and sensitivity of the biosensor for target DNA detection.

Main Methods:

  • Fabrication of a reflective microfiber Bragg grating (mFBG) in a silica microfiber.
  • Functionalization of the mFBG surface with poly-l-lysine (PLL) for DNA capture.
  • Monitoring the wavelength shifts of two distinct resonances in response to RI changes and temperature.
  • Real-time detection of DNA hybridization events and determination of the lowest detectable concentration.

Main Results:

  • The mFBG exhibited two well-defined resonances with differential RI response and identical temperature sensitivity.
  • Temperature-compensated RI measurements were successfully achieved by analyzing the wavelength separation.
  • The biosensor demonstrated real-time monitoring of surface functionalization and high specificity for DNA hybridization.
  • The lowest detectable concentration of target DNA was determined to be 0.5 µM.

Conclusions:

  • A label-free fiber-optic biosensor based on mFBG technology is effective for in-situ DNA hybridization detection.
  • The proposed sensor design enables accurate measurements by compensating for temperature fluctuations.
  • The biosensor shows high specificity and sensitivity, paving the way for advanced molecular diagnostics.