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Biodegradation of 2-hydroxyquinoxaline (2-HQ) by Bacillus sp
G V Subba Reddy1, B R Reddy2, M G Tlou3
1Department of Microbiology, Sri Krishnadevaraya University, Anantapuram - 515 003, A.P., India; Faculty of Science, Department of Biochemistry, University of Johannesburg, PO Box-524, APK Campus, Johannesburg 2006, South Africa.
Journal of Hazardous Materials
|June 24, 2014
Summary
A novel Bacillus strain, HQ2, was isolated from soil and effectively degrades 2-Hydroxyquinoxaline (2-HQ). This bacterium utilizes 2-HQ as its sole carbon and energy source, forming dimers during degradation.
Area of Science:
- Microbiology
- Environmental Science
- Biotechnology
Background:
- 2-Hydroxyquinoxaline (2-HQ) is a compound requiring microbial degradation.
- Isolation of specialized microorganisms is crucial for bioremediation.
Purpose of the Study:
- To isolate and characterize a bacterial strain capable of degrading 2-Hydroxyquinoxaline (2-HQ).
- To determine the optimal conditions for 2-HQ degradation by the isolated strain.
- To elucidate the degradation pathway of 2-HQ.
Main Methods:
- Isolation of bacteria from Chrysanthemum indicum soil.
- Identification using morphology, biochemical tests, and 16S rRNA sequencing.
- Optimization of degradation conditions (pH, temperature, inoculum density, substrate concentration).
- Gas Chromatography-Mass Spectrometry (GC-MS) for analyzing degradation products.
Main Results:
- An aerobic Gram-positive bacterium, identified as Bacillus sp. (strain HQ2), was isolated.
- Optimal degradation occurred at pH 6-8, 37-45 °C, with 1.0 OD inoculum, and 500 ppm 2-HQ.
- Nitrogen sources like yeast extract marginally enhanced degradation; carbon sources had no significant effect.
- GC-MS analysis revealed the formation of 2-HQ dimers during degradation, a novel finding.
Conclusions:
- Bacillus sp. strain HQ2 is an efficient degrader of 2-Hydroxyquinoxaline.
- The study reports a new degradation pathway involving dimer formation for 2-HQ.
- This finding has potential implications for bioremediation strategies involving quinoxaline compounds.
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