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Related Concept Videos

Protein Networks02:26

Protein Networks

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An organism can have thousands of different proteins, and these proteins must cooperate to ensure the health of an organism. Proteins bind to other proteins and form complexes to carry out their functions. Many proteins interact with multiple other proteins creating a complex network of protein interactions.
These interactions can be represented through maps depicting protein-protein interaction networks, represented as nodes and edges. Nodes are circles that are representative of a protein,...
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Protein Complexes with Interchangeable Parts01:57

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Protein Complexes with Interchangeable Parts01:57

Protein Complexes with Interchangeable Parts

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Groups of proteins may form a complex where each protein in this complex has a different role in the overall execution of the complex’s function. Often some of the proteins in the complex can be replaced by a closely related variant to give a complex that contains many of the same components yet is functionally distinct.
The SCF ubiquitin ligase is a protein complex of five individual proteins. This complex attaches ubiquitin to other target proteins to mark them for degradation. In order...
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Protein-protein Interfaces02:04

Protein-protein Interfaces

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Many proteins form complexes to carry out their functions, making protein-protein interactions (PPIs) essential for an organism's survival. Most PPIs are stabilized by numerous weak noncovalent chemical forces. The physical shape of the interfaces determines the way two proteins interact. Many globular proteins have closely-matching shapes on their surfaces, which form a large number of weak bonds. Additionally, many PPIs occur between two helices or between a surface cleft and a...
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Protein Complex Assembly02:41

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Proteins can form homomeric complexes with another unit of the same protein or heteromeric complexes with different types.  Most protein complexes self-assemble spontaneously via ordered pathways, while some proteins need assembly factors that guide their proper assembly. Despite the crowded intracellular environment, proteins usually interact with their correct partners and form functional complexes.
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Related Experiment Video

Updated: Apr 27, 2026

Resolving Affinity Purified Protein Complexes by Blue Native PAGE and Protein Correlation Profiling
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Resolving Affinity Purified Protein Complexes by Blue Native PAGE and Protein Correlation Profiling

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Structure-based characterization of multiprotein complexes.

Markus Wiederstein1, Markus Gruber1, Karl Frank1

  • 1Division of Structural Biology & Bioinformatics, Department of Molecular Biology, University of Salzburg, Hellbrunnerstraße 34, 5020 Salzburg, Austria.

Structure (London, England : 1993)
|June 24, 2014
PubMed
Summary
This summary is machine-generated.

We developed a fast search engine to find structural similarities in large protein complexes. This tool helps uncover previously unknown relationships in protein quaternary structure, aiding biological research.

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Area of Science:

  • Structural Biology
  • Computational Biology
  • Biochemistry

Background:

  • Cellular processes rely on multiprotein complexes.
  • Understanding their 3D structures is key to their biological function.
  • Identifying structural correlations requires extensive database searches.

Purpose of the Study:

  • To present a high-speed structure search engine for protein assemblies.
  • To enable instant matching of large protein oligomers against a comprehensive database.
  • To reveal and explore complex structural relationships in protein quaternary structure.

Main Methods:

  • Development of a high-speed structure search engine.
  • Utilizing a database of biologically functional protein assemblies.
  • Implementing structure-matching techniques for large protein oligomers.

Main Results:

  • The search engine provides instant matching capabilities.
  • Unseen structural correlations at the protein quaternary structure level were revealed.
  • Demonstrated the tool's effectiveness in exploring structural relationships.

Conclusions:

  • The developed search engine efficiently identifies structural correlations in protein assemblies.
  • Facilitates a deeper understanding of protein complex structure and function.
  • Offers a valuable tool for exploring complex structural relationships in structural biology.