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Direct visualization of the interrelationship between intramembrane and extracellular structures.

J B Wade1, R A Coleman

  • 1Department of Physiology, University of Maryland School of Medicine, Baltimore 21201.

Proceedings of the National Academy of Sciences of the United States of America
|April 1, 1989
PubMed
Summary

The toad urinary bladder

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Area of Science:

  • Cell Biology
  • Biophysics

Background:

  • The apical surface of toad urinary bladders features a glycocalyx mesh.
  • This mesh appears connected to the plasma membrane.

Purpose of the Study:

  • To investigate the relationship between the glycocalyx and intramembrane structures.
  • To visualize extracellular and membrane components simultaneously.

Main Methods:

  • Developed a composite replica technique.
  • Combined freeze-fracture for intramembrane visualization.
  • Used rotary shadowing after freeze-drying for glycocalyx replication.

Main Results:

  • Observed direct contacts between extracellular filaments and intramembrane particles.
  • Demonstrated a structural link between the glycocalyx and the plasma membrane.

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Conclusions:

  • The observed structural organization may stabilize membrane components.
  • This organization could anchor extracellular elements to the plasma membrane.