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Viable mononuclear cell stability study for implementation in a proficiency testing program: impact of shipment
Olga A Kofanova1, Kristine Davis, Barbara Glazer
11 Integrated BioBank Of Luxembourg (IBBL) , 6 rue Nicolas Ernest Barblé, L-1210, Luxembourg .
Biopreservation and Biobanking
|June 24, 2014
Summary
Shipping mononuclear cells requires careful temperature and media selection. Liquid nitrogen and dry ice with commercial solutions best preserve cell viability and function for proficiency testing.
Area of Science:
- Cell Biology
- Immunology
- Biotechnology
Background:
- Mononuclear cell viability is critical for research and diagnostics.
- Standardized shipping methods are needed for proficiency testing schemes.
Purpose of the Study:
- To evaluate the impact of shipping temperatures and preservation media on mononuclear cell viability and function.
- To identify optimal, cost-efficient shipping conditions for a proficiency testing scheme.
Main Methods:
- Peripheral blood mononuclear cells (PBMCs) and Jurkat cells were shipped under various conditions.
- Temperatures included ambient, dry ice, and liquid nitrogen.
- Preservation media tested: serum with cryoprotectant, commercial cryopreservation solution, and room temperature transport medium.
- Viability assessed by Trypan Blue, flow cytometry, and CASY; function by ELISpot assay.
Main Results:
- Liquid nitrogen shipment maintained the highest cell viability and functionality.
- Dry ice shipment and commercial cryopreservation solution offered stable, high-quality, and cost-efficient alternatives.
- Room temperature transport medium significantly compromised cell integrity.
Conclusions:
- Liquid nitrogen is optimal for preserving mononuclear cell viability and function.
- Dry ice shipment with commercial cryopreservation solutions provides a viable, cost-effective option for proficiency testing.
- Room temperature transport is unsuitable for maintaining mononuclear cell integrity.
