Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

The transcription factor OsbHLH55 regulates leaf inclination through the OsMAPK6-OsWRKY53 signaling pathway in rice.

Journal of experimental botany·2026
Same author

Synergism between SCT1/SCT2 and DNA methylation regulates the expression of Copia2 retrotransposon in rice.

Journal of integrative plant biology·2025
Same author

Evolutionary dynamics and genetic diversity of transposable elements revealed by resequencing data in maize population.

The Plant journal : for cell and molecular biology·2025
Same author

DNA Methylation in Rice: Mechanisms, Regulatory Roles, and Beyond.

International journal of molecular sciences·2025
Same author

Transcription factors ERF74/77/108/125 enhance thermotolerance in rice by regulating common and distinct heat-responsive gene expression.

Plant biotechnology journal·2025
Same author

Cadmium targeting transcription factor EB to inhibit autophagy-lysosome function contributes to acute kidney injury.

Journal of advanced research·2024

Related Experiment Video

Updated: Apr 27, 2026

Compact Quantum Dots for Single-molecule Imaging
17:14

Compact Quantum Dots for Single-molecule Imaging

Published on: October 9, 2012

17.5K

Microfluidic generation of multicolor quantum-dot-encoded core-shell microparticles with precise coding and enhanced

Yang Chen1, Peng-Fei Dong, Jian-Hong Xu

  • 1The State Key Lab of Chemical Engineering, Department of Chemical Engineering, Tsinghua University , Beijing 100084, China.

Langmuir : the ACS Journal of Surfaces and Colloids
|June 24, 2014
PubMed
Summary
This summary is machine-generated.

A new microfluidic method creates stable, multicolor quantum dot (QD)-encoded microparticles. This approach avoids QD leakage and simplifies multicolor analysis without complex algorithms.

More Related Videos

Production and Targeting of Monovalent Quantum Dots
10:16

Production and Targeting of Monovalent Quantum Dots

Published on: October 23, 2014

26.4K
Fabrication of Spherical and Worm-shaped Micellar Nanocrystals by Combining Electrospray, Self-assembly, and Solvent-based Structure Control
06:16

Fabrication of Spherical and Worm-shaped Micellar Nanocrystals by Combining Electrospray, Self-assembly, and Solvent-based Structure Control

Published on: February 11, 2018

20.5K

Related Experiment Videos

Last Updated: Apr 27, 2026

Compact Quantum Dots for Single-molecule Imaging
17:14

Compact Quantum Dots for Single-molecule Imaging

Published on: October 9, 2012

17.5K
Production and Targeting of Monovalent Quantum Dots
10:16

Production and Targeting of Monovalent Quantum Dots

Published on: October 23, 2014

26.4K
Fabrication of Spherical and Worm-shaped Micellar Nanocrystals by Combining Electrospray, Self-assembly, and Solvent-based Structure Control
06:16

Fabrication of Spherical and Worm-shaped Micellar Nanocrystals by Combining Electrospray, Self-assembly, and Solvent-based Structure Control

Published on: February 11, 2018

20.5K

Area of Science:

  • Materials Science
  • Nanotechnology
  • Biotechnology

Background:

  • Quantum dots (QDs) are widely used in bioassays and imaging due to their unique optical properties.
  • Developing stable and precisely encoded multicolor microparticles is crucial for multiplexed assays.
  • Existing methods for multicolor QDs often face challenges with photostability and spectral overlap.

Purpose of the Study:

  • To develop a novel microfluidic approach for preparing multicolor QDs-encoded core-shell microparticles.
  • To enhance the stability and coding precision of QDs within microparticles.
  • To simplify multicolor detection by avoiding spectral deconvolution.

Main Methods:

  • Utilized a microfluidic system to fabricate core-shell microparticles.
  • Encapsulated different types of QDs into distinct core structures within the microparticles.
  • Incorporated a hydrogel shell to protect the QDs and enhance stability.

Main Results:

  • Achieved precise and diverse barcode patterns in multicolor QDs-encoded microparticles.
  • Demonstrated significantly enhanced fluorescent stability due to the hydrogel shell, preventing QD leakage.
  • Eliminated the need for spectral deconvolution algorithms by separating QDs into different cores, allowing individual fluorescence recording.

Conclusions:

  • The developed microfluidic method offers a robust platform for creating highly stable and precisely encoded multicolor QDs-encoded microparticles.
  • This approach overcomes limitations of traditional QD mixtures, enabling accurate multiplexed detection even with spectrally similar QDs.
  • The core-shell structure provides enhanced photostability and simplifies data analysis in various bioassay applications.