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[Effect and mechanism of polyphyllin I on human cervical cancer cell HeLa in vitro]
Objective:
To study the effect and mechanism of polyphyllin I on human cervical cancer cell HeLa.
Methods:
The cell growth and proliferation effect of Polyphyllin I on HeLa cells were measured by MTT assay; Hoechst 33258 fluorescent staining was used to record changes in cell morphology and morphological changes in mitochondria of Polyphyllin I before and after treatment on He La cells. Annexin V-FITC/PI staining was used to detect the ratio of tumor cell apoptosis by flow cytometry. Release of intracellular re active oxygen species (ROS) generation level in HeLa cells was determined by flow cytometry,Caspase-3 activity was measured by fluorescent assay kits.
Results:
MTT results showed that Polyphyllin I could significantly suppressed the proliferation of HeLa cells and in time-and concentration-dependence manner. The intracellular ROS levels were increased dramatically and the mitochondrial membrane was decreased consistently. Caspase-3 proteins expression levels were increased after Polyphyllin I treatment.
Conclusion:
Polyphyllin I could inhibit HeLa cells growth and proliferation and its mechanism may be related to inducing cell apoptosis.
