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Related Concept Videos

Antigens Involved in Adaptive Immunity01:26

Antigens Involved in Adaptive Immunity

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An antigen is any substance the immune system identifies as foreign and potentially harmful to the body, prompting an immune response. Antigens have two functional properties: immunogenicity and reactivity. Immunogenicity is the ability of an antigen to stimulate a specific immune response. At the same time, reactivity describes the antigen's ability to react with the cells and antibodies produced in response to it.
Complete Antigens
Complete antigens possess both immunogenicity and...
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Immune Response Against Viral Pathogens01:29

Immune Response Against Viral Pathogens

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The immune system's response to viral infections is a complex and coordinated process involving natural killer (NK) cells, T cell-mediated responses, and antibody-mediated responses.
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Antigen Processing Pathways01:31

Antigen Processing Pathways

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MHC molecules are key players in the immune response, enabling T cells to recognize and respond to specific antigens. They are present on the surface of all nucleated cells in the body and are instrumental in presenting antigens to T cells and activating them. T cells recognize the MHC-antigen complex and initiate an immune response. MHC class I and MHC class II are two main types of MHC molecules, each associated with a distinct antigen processing pathway.
MHC Class I: Presenting Endogenous...
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Antigen Presenting Cells01:22

Antigen Presenting Cells

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The immune system is a complex network of cells and molecules that protects the body from foreign invaders. T cells, a type of white blood cell, play a crucial role in this process. They recognize and attack foreign substances, such as pathogens, that enter the body.
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T Cell Activation and Clonal Selection01:22

T Cell Activation and Clonal Selection

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T cells are integral to our adaptive immune system, recognizing and effectively responding to foreign antigens. T cell activation and clonal selection are pivotal in orchestrating this immune response. This article elucidates these mechanisms, detailing the roles of cluster of differentiation (CD) markers, major histocompatibility complex (MHC) molecules, costimulatory signals, and the process of clonal selection.
Naive T cells that have not yet encountered an antigen express two primary CD...
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Cells of the Innate Immune Response01:28

Cells of the Innate Immune Response

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The innate immune response is an immediate and non-specific response against pathogens, acting swiftly to prevent the spread of infections. The primary cells involved in this response are phagocytes and natural killer (NK) cells.
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Related Experiment Video

Updated: Apr 27, 2026

Chemical Conjugation of a Purified DEC-205-Directed Antibody with Full-Length Protein for Targeting Mouse Dendritic Cells In Vitro and In Vivo
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Chemical Conjugation of a Purified DEC-205-Directed Antibody with Full-Length Protein for Targeting Mouse Dendritic Cells In Vitro and In Vivo

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Nucleic acid recognition in dendritic cells.

Alexander Heiseke1, Katharina Eisenächer, Anne Krug

  • 1II. Medical Department, Klinikum rechts der Isar, Technical University Munich, Ismaningerstr. 22, 81675, Munich, Germany.

Methods in Molecular Biology (Clifton, N.J.)
|June 25, 2014
PubMed
Summary

This study details methods for generating dendritic cells (DCs) from human and mouse cells. These professional antigen-presenting cells (APCs) are crucial for bridging innate and adaptive immunity.

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Area of Science:

  • Immunology
  • Cell Biology
  • Molecular Biology

Background:

  • The immune system relies on specialized cells, including dendritic cells (DCs), for defense against pathogens.
  • DCs are professional antigen-presenting cells (APCs) that link innate and adaptive immunity.
  • Recognition of pathogens via pattern recognition receptors (PRRs) on DCs initiates immune responses.

Purpose of the Study:

  • To provide detailed protocols for generating various types of dendritic cells (DCs).
  • To outline methods for stimulating DCs using pattern recognition receptor (PRR) ligands in vitro.
  • To facilitate research on DC function and their role in immune responses.

Main Methods:

  • Isolation and differentiation of dendritic cells (DCs) from human peripheral blood mononuclear cells (PBMCs).
  • Generation of dendritic cells (DCs) from murine bone marrow.
  • In vitro stimulation of DCs using pattern recognition receptor (PRR) ligands.

Main Results:

  • Established protocols for generating functional dendritic cells (DCs) from human and murine sources.
  • Demonstrated effective activation of DCs using specific PRR ligands.
  • Provided a foundation for studying DC-mediated antigen presentation and T cell activation.

Conclusions:

  • Dendritic cells (DCs) are essential for initiating adaptive immune responses.
  • The provided methods enable the generation and activation of DCs for in vitro studies.
  • These protocols support research into the critical role of DCs in bridging innate and adaptive immunity.