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Mapping of optimal CD8 T cell epitopes

Julia Roider1, Thomas Vollbrecht, Rika Draenert

  • 1Department of infectious diseases, Medizinische Klinik und Poliklinik IV, Klinikum der Universität München, Campus Innenstadt, Pettenkoferstraße 8a, 80336, Munich, Germany.

Methods in Molecular Biology (Clifton, N.J.)
|June 25, 2014
PubMed

Defining the optimal epitope of a CD8 T cell response towards a certain antigen is a multistep procedure that requires the performance of peptide truncation design, ELISPOT peptide titration assays, and assessing the HLA class I restriction of the defined epitope via intracellular cytokine staining assays with B cell lines and epitope-specific CD8 T cell lines.

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