Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

A Sustainable Organic Memristor with Tunable Switching for Logic Operations and Medical Image Encryption.

Small (Weinheim an der Bergstrasse, Germany)·2026
Same author

Adaptable Covalent Organic Framework-Hydrogel Microneedles for Glucose-Responsive Isoliquiritigenin Delivery in Diabetic Wound Therapy.

Advanced healthcare materials·2026
Same author

Tumour-Derived Extracellular Vesicles Induce Diffuse Large B-Cell Lymphoma Progression Through Macrophage-Mediated MDSC Accumulation.

Journal of extracellular vesicles·2026
Same author

Immunomodulatory carbon dots balance the anti-inflammatory, antibacterial, and osteogenic activities of metal-phenolic network for osteomyelitis therapy.

Biomaterials·2026
Same author

DLP-bioprinted ultrabiomimetic trachea with spatiotemporal angiogenesis regulation for segmental airway reconstruction.

Science advances·2026
Same author

StLAX5 regulates stolon initiation to control the tuber number and productivity of potato.

The New phytologist·2026

Related Experiment Video

Updated: Apr 27, 2026

Multispectral Real-time Fluorescence Imaging for Intraoperative Detection of the Sentinel Lymph Node in Gynecologic Oncology
06:37

Multispectral Real-time Fluorescence Imaging for Intraoperative Detection of the Sentinel Lymph Node in Gynecologic Oncology

Published on: October 20, 2010

23.8K

Dextran-based fluorescent nanoprobes for sentinel lymph node mapping.

Tingting Dai1, Shuyan Zhou2, Chuyang Yin3

  • 1Department of Plastic and Reconstructive Surgery, Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai Key Laboratory of Tissue Engineering, National Tissue Engineering Center of China, 639 Zhi Zao Ju Road, Shanghai 200011, PR China.

Biomaterials
|June 25, 2014
PubMed
Summary

Researchers developed a novel fluorescent dextran-based nanogel (FDNG) for precise sentinel lymph node (SLN) mapping. This biosafe imaging agent offers high sensitivity and stability, aiding in tumor metastasis prediction and guiding therapies.

Keywords:
BiosafetyFluorescent dextran-based nanogelsLymphatic endothelial cellsMappingSentinel lymph node

More Related Videos

The Application of 1% Methylene Blue Dye As a Single Technique in Breast Cancer Sentinel Node Biopsy
07:51

The Application of 1% Methylene Blue Dye As a Single Technique in Breast Cancer Sentinel Node Biopsy

Published on: June 1, 2019

21.2K
Computed Tomography-guided Time-domain Diffuse Fluorescence Tomography in Small Animals for Localization of Cancer Biomarkers
12:24

Computed Tomography-guided Time-domain Diffuse Fluorescence Tomography in Small Animals for Localization of Cancer Biomarkers

Published on: July 17, 2012

13.2K

Related Experiment Videos

Last Updated: Apr 27, 2026

Multispectral Real-time Fluorescence Imaging for Intraoperative Detection of the Sentinel Lymph Node in Gynecologic Oncology
06:37

Multispectral Real-time Fluorescence Imaging for Intraoperative Detection of the Sentinel Lymph Node in Gynecologic Oncology

Published on: October 20, 2010

23.8K
The Application of 1% Methylene Blue Dye As a Single Technique in Breast Cancer Sentinel Node Biopsy
07:51

The Application of 1% Methylene Blue Dye As a Single Technique in Breast Cancer Sentinel Node Biopsy

Published on: June 1, 2019

21.2K
Computed Tomography-guided Time-domain Diffuse Fluorescence Tomography in Small Animals for Localization of Cancer Biomarkers
12:24

Computed Tomography-guided Time-domain Diffuse Fluorescence Tomography in Small Animals for Localization of Cancer Biomarkers

Published on: July 17, 2012

13.2K

Area of Science:

  • Biomedical Engineering
  • Nanotechnology
  • Medical Imaging

Background:

  • Sentinel lymph node (SLN) biopsy is crucial for predicting tumor metastasis.
  • Current SLN tracers have limitations, necessitating the development of improved imaging agents.
  • Accurate SLN mapping requires tracers with high specificity, sensitivity, and biosafety.

Purpose of the Study:

  • To design and evaluate a novel fluorescent nanoprobe for accurate sentinel lymph node (SLN) mapping.
  • To assess the biosafety and biocompatibility of the developed imaging agent.
  • To explore the potential of the nanogel platform for drug delivery applications.

Main Methods:

  • Fabrication of dextran-based nanogel (DNG) via self-assembly.
  • Conjugation of fluorescent agents to DNG to create fluorescent DNG (FDNG).
  • In vitro and in vivo evaluation of FDNG for SLN imaging, including dynamic course studies and biosafety assessments.

Main Results:

  • FDNG demonstrated optimal size and stable fluorescent intensity for SLN imaging.
  • In vivo studies showed satisfactory specificity, sensitivity, and stability for SLN mapping over 72 hours.
  • FDNG exhibited excellent biosafety and biocompatibility with lymphatic endothelial cells.

Conclusions:

  • FDNG serves as a highly efficient molecular imaging probe for non-invasive and safe SLN mapping.
  • FDNG provides accurate localization for SLN biopsy, aiding in tumor metastasis prediction and therapy guidance.
  • The DNG platform shows potential as a drug carrier for lymph-associated diseases.