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Untargeted Liquid Chromatography-Mass Spectrometry-Based Metabolomics Analysis of Wheat Grain
Published on: March 13, 2020
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Profiling of mitochondrial proteome in wheat roots
Da-Eun Kim1, Swapan Kumar Roy, Abu Hena Mostafa Kamal
1Department of Crop Science, Chungbuk National University, 410 Seongbong-ro, Heungdeok-gu, Cheongju, Chungbuk, 361-763, Korea.
Molecular Biology Reports
|June 25, 2014
Summary
This study systematically analyzed the mitochondrial proteome in wheat roots, identifying 184 proteins involved in crucial cellular functions like energy metabolism and translation. These findings offer insights into wheat root mitochondrial protein composition.
Area of Science:
- Plant Biology
- Cellular Biology
- Proteomics
Background:
- Mitochondria are vital organelles in eukaryotic cells, essential for cellular respiration.
- They play key roles in various cellular processes, including vitamin synthesis and amino acid metabolism.
Purpose of the Study:
- To systematically investigate and characterize the mitochondrial proteome in wheat seedling roots.
- To identify and functionally classify proteins within wheat root mitochondria.
Main Methods:
- Mitochondria were isolated from 15-day-old wheat seedling roots using Percoll gradient centrifugation.
- Protein extraction, dissociation, and analysis were performed using Tricine SDS-PAGE coupled with LTQ-FTICR mass spectrometry.
Main Results:
- A total of 184 proteins were identified, with 140 confirmed as mitochondrial and 44 predicted as other subcellular proteins.
- Identified mitochondrial proteins were functionally classified into 12 categories, with significant representation in energy metabolism (20%) and translation (22.1%).
Conclusions:
- The identified wheat root mitochondrial proteome shares common protein components and functions with previously studied mitochondrial proteomes.
- This study provides extensive data on the mitochondrial proteins of wheat roots, contributing novel insights into their composition and function.
