Introduction of macromolecules into primary cultured neuronal cells by fusion with erythrocyte ghosts

The macromolecules were injected into primary cultured neuronal cells by fusion with human erythrocyte ghosts containing macromolecules. The injection efficiency was about 30% and 80% of the cells extended neurites like normal neuronal cells. In order to succeed in this method, phytohaemagglutinin E and polyethylene glycol 1000 were essential, and monolayer culture cells were used.