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Induction of Murine Intestinal Inflammation by Adoptive Transfer of Effector CD4+CD45RBhigh T Cells into Immunodeficient Mice
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PPARβ/δ activation of CD300a controls intestinal immunity

Toshiya Tanaka1, Satoko Tahara-Hanaoka2, Tsukasa Nabekura2

  • 1Laboratory for Systems Biology and Medicine (LSBM), Research Center for Advanced Science and Technology (RCAST), The University of Tokyo, Tokyo 153-8904, Japan.

Scientific Reports
|June 25, 2014
PubMed

Insights

CD300a deficiency in macrophages disrupts intestinal immune homeostasis, leading to inflammation and malabsorption, particularly on a high-fat diet. This highlights CD300a

Area of Science:

  • Immunology
  • Gastroenterology
  • Cell Biology

Background:

  • Macrophages play a crucial role in maintaining intestinal immune balance.
  • Peroxisome proliferator-activated receptor beta/delta (PPARβ/δ) influences immune responses.
  • CD300a is an immunoreceptor tyrosine-based inhibitory motif (ITIM)-containing receptor on immune cells.

Purpose of the Study:

  • To investigate the role of PPARβ/δ in regulating CD300a expression in macrophages.
  • To determine the impact of CD300a deficiency on intestinal immunity and metabolism under high-fat diet conditions.

Main Methods:

  • Utilized knockout mouse models lacking CD300a.
  • Administered high-fat diet (HFD) to assess metabolic and inflammatory responses.
  • Performed bone marrow transplantation to confirm the cellular origin of observed phenotypes.
  • Analyzed macrophage activation states (M1) and cytokine secretion (IL-6) following TLR4/MyD88 pathway stimulation.

Main Results:

  • CD300a deficiency in mice on HFD resulted in chronic intestinal inflammation, reduced lymph capillaries, and enlarged mesenteric lymph nodes.
  • Mice lacking CD300a exhibited triglyceride malabsorption and impaired weight gain on HFD.
  • Macrophages from CD300a-deficient mice showed enhanced M1 activation and prolonged IL-6 secretion upon LPS stimulation.
  • Phenotypic changes were attributed to CD300a deficiency in leukocytes.

Conclusions:

  • PPARβ/δ directly regulates CD300a in macrophages.
  • CD300a-mediated inhibitory signaling is critical for regulating intestinal immune homeostasis.
  • CD300a deficiency exacerbates diet-induced intestinal inflammation and metabolic dysfunction.

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