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Bacterial Phylum Chlamydiae01:29

Bacterial Phylum Chlamydiae

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The phylum Chlamydiae or Chlamydiota is composed of a single order, Chlamydiales. This phylum consists entirely of obligate intracellular parasites that infect eukaryotic hosts. While human pathogens within this group have been studied extensively, the phylum encompasses many species capable of interacting with various eukaryotic organisms. Members of Chlamydiae are typically small cocci, approximately 0.5 μm in diameter, and exhibit a distinctive developmental cycle. As is characteristic...
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A Human Fallopian Tube Model for Investigation of C. trachomatis Infections
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Human enteroendocrine cell responses to infection with Chlamydia trachomatis: a microarray study.

Aldona Dlugosz1, Sandra Muschiol2, Katherina Zakikhany3

  • 1Karolinska Institutet, Department of Medicine, Division of Gastroenterology and Hepatology, Karolinska University Hospital, Gastrocentrum Huddinge K63, Stockholm, Sweden.

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|June 25, 2014
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Chlamydia trachomatis infection alters gene expression in human enteroendocrine cells (EEC), impacting vesicular transport and secretion. This may contribute to gut motility disorders.

Keywords:
Chlamydia trachomatisEnteroendocrine cellsInfectionMicroarray

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Area of Science:

  • Gastroenterology
  • Cell Biology
  • Infectious Disease

Background:

  • Enteroendocrine cells (EEC) produce vital gut signaling molecules.
  • Previous work indicated altered protein distribution in Chlamydia-infected EEC.
  • This study investigates EEC response to Chlamydia infection in vitro.

Purpose of the Study:

  • To perform microarray analysis of EEC response to Chlamydia trachomatis infection.
  • To identify changes in gene expression in both large and small bowel EEC.
  • To model Chlamydia infection in human EEC lines.

Main Methods:

  • Utilized two human EEC lines (LCC-18 and CNDT-2).
  • Infected cells with Chlamydia trachomatis (serovar LGV II strain 434).
  • Employed microarray analysis to study gene expression changes during active and persistent infection.

Main Results:

  • Significant changes in gene expression observed in both cell lines post-infection.
  • Genes involved in apoptosis, cell differentiation, and vesicular transport were affected.
  • Specific differences noted in solute carrier transporters (SLC) and endocrine-related genes.

Conclusions:

  • Infected EEC display cell-type specific patterns in vesicular transport, secretion, and neurotransmitter regulation.
  • Impaired enteroendocrine function due to infection can contribute to gut motility disorders.
  • EEC play a critical role in regulating gut motility.