Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Disorders of Leukocytes01:27

Disorders of Leukocytes

2.3K
Leukocyte disorders can lead to either leukopenia, characterized by an abnormally low leukocyte count, or leukocytosis, marked by a very high leukocyte number.
Leukopenia may result from bone marrow disorders, autoimmune diseases, and infectious diseases. For example, conditions such as multiple myeloma and aplastic anemia can impair the bone marrow's ability to produce adequate leukocytes. Similarly, autoimmune diseases like lupus and viral infections such as HIV can prompt the immune...
2.3K
Differentiation of Common Myeloid Progenitor Cells01:15

Differentiation of Common Myeloid Progenitor Cells

3.1K
Common myeloid progenitors (CMPs) are oligopotent cells that can differentiate into granulocytes and macrophages. Granulocytes and macrophages are essential for protecting the body against bacterial, viral, or fungal infections. They migrate from the bone marrow into the circulating blood to reach specific tissue sites where they differentiate and help in immune surveillance. However, they survive only for a few days and must be continuously made available to the organism to maintain a robust...
3.1K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Phenanthroline-Functionalized Ligand for Constructing CuBr-Modified Zinc-Organic Framework for Cyclization of Propargylic Amines with Flue Gas at Ambient Conditions.

Inorganic chemistry·2026
Same author

Sensitive fluorescent europium-organic framework sensor for selective discrimination of tetracycline and norfloxacin and applications in food samples.

Food chemistry·2026
Same author

How glycemic variability trajectories impact clinical outcomes in critically ill patients with severe pneumonia: a prospective cohort study.

BMC anesthesiology·2026
Same author

Architecting Silver-Organic Frameworks Based on Tetraimidazole-Functionalized Spirofluorene for the Cyclization of Propargylic Amines with CO<sub>2</sub> under Ambient Conditions.

Inorganic chemistry·2026
Same author

Taxonomic novelties in <i>Helminthosphaeriaceae</i>: morphological and phylogenetic evidence for <i>Obovatispora</i> gen. nov. and a new species from <i>Polygonatum</i> in Guizhou Province, China.

MycoKeys·2026
Same author

Visible Light-Driven Singlet Oxygen-Mediated Aerobic Oxidation to Synthesize Quinazolinones Catalyzed by a Photosensitizing [Th<sub>6</sub>]-Cluster-Based Metal-Organic Framework.

Inorganic chemistry·2026

Related Experiment Video

Updated: Apr 27, 2026

Comprehensive Protocol to Sample and Process Bone Marrow for Measuring Measurable Residual Disease and Leukemic Stem Cells in Acute Myeloid Leukemia
09:57

Comprehensive Protocol to Sample and Process Bone Marrow for Measuring Measurable Residual Disease and Leukemic Stem Cells in Acute Myeloid Leukemia

Published on: March 5, 2018

32.1K

Difference in glycerol levels between leukemia and normal bone marrow stem cells.

Ying-Song Qin1, Dan-Xia Bu1, Ying-Ying Cui2

  • 1Department of Pathophysiology, Xuzhou Medical College, Xuzhou, Jiangsu 221002, P.R. China.

Oncology Letters
|June 25, 2014
PubMed
Summary

Intracellular glycerol levels are higher in hematopoietic stem cells (HSCs), contributing to leukemia, but aquaglyceroporin 9 (AQP9) is not involved. This finding aids understanding leukemia carcinogenesis and developing therapies.

Keywords:
AQP9glycerinleukemiastem cells

More Related Videos

Assessment of the Metabolic Profile of Primary Leukemia Cells
06:21

Assessment of the Metabolic Profile of Primary Leukemia Cells

Published on: November 21, 2018

9.7K
Author Spotlight: Analyzing Bone Marrow Microenvironment in Murine Hematological Malignancies
06:33

Author Spotlight: Analyzing Bone Marrow Microenvironment in Murine Hematological Malignancies

Published on: November 10, 2023

2.0K

Related Experiment Videos

Last Updated: Apr 27, 2026

Comprehensive Protocol to Sample and Process Bone Marrow for Measuring Measurable Residual Disease and Leukemic Stem Cells in Acute Myeloid Leukemia
09:57

Comprehensive Protocol to Sample and Process Bone Marrow for Measuring Measurable Residual Disease and Leukemic Stem Cells in Acute Myeloid Leukemia

Published on: March 5, 2018

32.1K
Assessment of the Metabolic Profile of Primary Leukemia Cells
06:21

Assessment of the Metabolic Profile of Primary Leukemia Cells

Published on: November 21, 2018

9.7K
Author Spotlight: Analyzing Bone Marrow Microenvironment in Murine Hematological Malignancies
06:33

Author Spotlight: Analyzing Bone Marrow Microenvironment in Murine Hematological Malignancies

Published on: November 10, 2023

2.0K

Area of Science:

  • Hematology
  • Molecular Biology
  • Oncology

Background:

  • Aquaglyceroporin 9 (AQP9) is implicated in carcinogenesis, notably liver carcinoma, with decreased expression linked to apoptosis resistance.
  • The role of AQP9 in leukemia, particularly its involvement with hematopoietic stem cells (HSCs), remains unclear.
  • Abnormal HSC proliferation is a proposed mechanism in leukemia development.

Purpose of the Study:

  • To investigate the potential roles of AQP9 in HSCs.
  • To determine the effect of AQP9 on intracellular glycerol content within HSCs.
  • To explore the relationship between AQP9, glycerol, and leukemia carcinogenesis.

Main Methods:

  • Isolation of HSCs and non-HSCs (nHSCs) using magnetic-activated cell sorting and flow cytometry.
  • Assessment of AQP9 expression at mRNA and protein levels via RT-PCR and western blotting.
  • Quantification of intracellular glycerol content using ELISA and morphological analysis via Wright-Giemsa staining.

Main Results:

  • HSCs exhibited significantly higher intracellular glycerol content compared to nHSCs.
  • AQP9 mRNA and protein expression were undetectable in both HSCs and nHSCs but present in white blood cells (WBCs).
  • HSC morphology was characterized by specific cellular and nuclear features, with nuclei occupying a large portion of the cell.

Conclusions:

  • Intracellular glycerol plays a role in HSC proliferation, independent of AQP9 mediation.
  • The findings suggest AQP9 is not directly involved in glycerol transport in HSCs relevant to leukemia.
  • This research provides insights into leukemia carcinogenesis mechanisms and potential therapeutic targets.