Related Experiment Video
Updated: Apr 27, 2026

Sampling and Pretreatment of Tooth Enamel Carbonate for Stable Carbon and Oxygen Isotope Analysis
Published on: August 15, 2018
Human age estimation from tooth cementum and dentin
Priya Gupta1, Harshaminder Kaur2, Madhu Shankari G S3
1Senior Lecturer, Department of Oral and Maxillofacial Pathology, Luxmi Bai Institute of Dental Sciences and Hospital , Patiala, Punjab, India .
Background:
Age estimation is an important factor in the identification of an individual in forensic science. The hard tissues of the human dentition are able to resist decay and degradation, long after other tissues are lost. This resistance has made teeth useful indicators for age calculation. Recent research has indicated that Tooth Cementum Annulations (TCA) may be used more reliably than any other morphological or histological traits of the adult skeleton, for age estimation.
Aims:
The purpose of this study was to examine the correlation between age and the number of incremental lines in human cementum and to correlate age with thickness of secondary dentin.
Materials And Methods:
The study sample consisted of 100 teeth. Teeth which were extracted because of periodontal disease and orthodontic, and prosthetic reasons were used in the study. The exclusion criterion was teeth with carious lesions. The age of the individuals at the time of tooth extraction ranged from 25-60 years. Longitudinal ground sections of each tooth were prepared and examined.
Statistical Analysis:
Correlation between estimated age, which was calculated by using cemental lines and thicknes s of secondary dentin and actual age, was found by using Pearson's correlation coefficient. Correlation of increase in thickness of secondary dentin in different age groups was analyzed by ANOVA test.
Results:
A strong positive correlation was found between the estimated age, which was calculated by using cemental lines and thickness of secondary dentin and actual age. Correlation of increase in thickness of secondary dentin in different age groups was found to be non significant.
Conclusion:
Countable cemental annulations are present in human teeth. Quantification of cementum annuli is a moderately reliable means which is used for age estimation in humans. As the age advances, the thickness of the secondary dentin also increases; hence, the amount of secondary dentin can also be an indicator of age of an individual.
Related Concept Videos
Teeth
In the bud stage, the tooth germ (an aggregation of cells) starts to form in the developing jawbone. During the cap stage, the tooth germ differentiates into enamel organ, dental papilla, and dental sac, which will later develop into the tooth's enamel, dentin...
Tooth Anatomy
The Crown, Neck, and Root
The visible part of the tooth is referred to as the crown. It's covered by enamel, the hardest substance in the human body. The crown is uniquely shaped for each type of tooth, allowing for different functions such as cutting, tearing, or...
Radioactive Decay and Radiometric Dating

