Related Experiment Video
Updated: Apr 27, 2026
Evaluation of Polymeric Gene Delivery Nanoparticles by Nanoparticle Tracking Analysis and High-throughput Flow Cytometry
Published on: March 1, 2013
Efficient targeted gene delivery by a novel PAMAM/DNA dendriplex coated with hyaluronic acid
Koldo Urbiola1, Carmen Sanmartín, Laura Blanco-Fernández
1Department of Pharmacy & Pharmaceutical Technology, School of Pharmacy, University of Navarra, C/Irunlarrea 1, 31080 Pamplona, Spain.
Aim:
To design and develop a novel target-specific DNA-delivery system using hyaluronic acid (HA)-polyamidoamine (PAMAM) conjugates (P-HA).
Materials & Methods:
The coupling of HA to the PAMAM dendrimer was analyzed by (1)H-NMR and elemental analysis (CHN). Their properties were characterized in terms of size and zeta-potential and evaluated for in vitro and in vivo transfection efficiency.
Results:
The designed covalent HA-dendriplexes enhanced gene transfection of pCMV-Luc reporter gene in overexpressing CD44-receptor cancer cells. They were also more efficient in transfecting MDA-MB231 cells than conventional PEI-polyplexes. The cytotoxicity of the covalent HA-dendriplexes was lower than when using conventional polyethylenimine-polyplexes. In vivo studies showed that these targeted complexes were also efficient for delivering pCMVLuc in different organs of healthy mice, as well as in tumors of C57BL/6 animals.
Conclusions:
The HA-dendriplexes developed in this work may offer an advantageous alternative to conventional cationic polymer-based formulations for DNA delivery into cancer cells in an efficient and safe manner.
More Related Videos
08:09Methionine Functionalized Biocompatible Block Copolymers for Targeted Plasmid DNA Delivery
Published on: August 6, 2019
09:56Epigenetic Engineering of K562 Cells: Dual-Vector Episomal Strategy for Stable Targeted DNA Methylation using dCas9-DNMT3A and -HDAC1 Fusion Proteins
Published on: October 31, 2025
