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Fluorescence detection of DNA hybridization based on the aggregation-induced emission of a perylene-functionalized
Guojie Wang1, Ruichen Zhang, Chen Xu
1School of Materials Science and Engineering, University of Science and Technology Beijing , Beijing 100083, China.
Abstract:
A perylene-functionalized polycation was synthesized by quaternization of poly(4-vinylpyridine) with bromomethyl-perylene and methyl iodide, which exhibited a unique aggregation-induced emission (AIE) effect. The synthesized polycation and polyanion ssDNA could form a complex probe to detect DNA hybridization. Upon adding noncomplementary ssDNA, the fluorescence of the complex probe increased due to the AIE effect; upon adding complementary ssDNA, the fluorescence intensity changed little due to the combined effects of AIE and duplex-quenching resulting from the intercalation of perylene into the duplex.
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