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Patch Clamp

Patch Clamp

Many fundamental cell functions such as muscle contraction and nerve transmission rely on the electrical signals produced by the movement of positively and negatively charged ions across the cell membrane. One competent method to record current flowing across the whole cell or single ion channel is the patch-clamp technique.
In this method, a glass micropipette containing electrolyte solution is tightly sealed against a small portion of the cell membrane. As a result, a patch of the cell...

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Related Experiment Video

Updated: Apr 27, 2026

One-channel Cell-attached Patch-clamp Recording

One-channel Cell-attached Patch-clamp Recording

Published on: June 9, 2014

One-channel cell-attached patch-clamp recording.

Bruce A Maki¹, Kirstie A Cummings², Meaghan A Paganelli¹

¹Graduate Program in Neuroscience, SMBS, University at Buffalo, SUNY.

Journal of Visualized Experiments : Jove

|June 26, 2014

Summary

This summary is machine-generated.

This study details a cell-attached patch-clamp method for recording single ion channel activity. This technique reveals ion channel dynamics and gating mechanisms, aiding in understanding their roles in health and disease.

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Last Updated: Apr 27, 2026

One-channel Cell-attached Patch-clamp Recording

One-channel Cell-attached Patch-clamp Recording

Published on: June 9, 2014

Patch Clamp Recording of Ion Channels Expressed in Xenopus Oocytes

Patch Clamp Recording of Ion Channels Expressed in Xenopus Oocytes

Published on: October 16, 2008

Whole-cell Patch-clamp Recordings for Electrophysiological Determination of Ion Selectivity in Channelrhodopsins

Whole-cell Patch-clamp Recordings for Electrophysiological Determination of Ion Selectivity in Channelrhodopsins

Published on: May 22, 2017

Area of Science:

Biophysics
Neuroscience
Molecular Biology

Background:

Ion channels are crucial for cellular communication across membranes.
Understanding ion channel dynamics is key to current research.
Single-molecule recordings offer insights into channel operational dynamics.

Purpose of the Study:

To describe a protocol for one-channel cell-attached patch-clamp recordings.
To analyze ligand-gated (NMDA receptor) and mechanosensitive (PIEZO1) ion channels.
To provide data on conductance properties and open-closed conformations.

Main Methods:

Utilizing cell-attached patch-clamp technique.
Recording single-channel currents from NMDA receptors in HEK293 cells and neurons.
Adapting the method for other channels like PIEZO1.

Main Results:

Successful acquisition of single-channel current recordings.
Characterization of channel conductance properties.
Analysis of temporal sequences of channel gating (open-closed conformations).

Conclusions:

The described method provides valuable data on ion channel function.
This technique aids in understanding ion channel mechanisms in physiological and pathological states.
The protocol is adaptable for studying diverse ion channels.