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Differential induction of macrophage GSIB4-binding activity
D R Tabor1, C H Larry, R F Jacobs
1Department of Pediatrics, University of Arkansas for Medical Sciences, Little Rock 72205-7199.
Journal of Leukocyte Biology
|May 1, 1989
Summary
Activated macrophages express a marker that binds Griffonia simplicifolia-IB4 (GSIB4) lectin. Various stimuli, including gamma-interferon and phagocytosis, enhance this GSIB4 binding, indicating increased macrophage responsiveness.
Area of Science:
- Immunology
- Cell Biology
Background:
- Activated macrophages possess a terminal galactopyranosyl group on their surface.
- This group binds the Griffonia simplicifolia-IB4 (GSIB4) lectin, serving as a potential activation marker.
Purpose of the Study:
- To investigate the induction and expression of GSIB4 binding on peritoneal macrophages.
- To determine how different stimuli influence GSIB4 binding and macrophage responsiveness.
Main Methods:
- Utilized FITC-conjugated GSIB4 to assess marker expression on normal (NMO) and LPS-treated (LPS MO) mouse peritoneal macrophages.
- Examined the effects of in vitro culture, latex bead phagocytosis, and gamma-interferon (gamma-IFN) treatment on GSIB4 binding.
Main Results:
- LPS MO exhibited higher baseline GSIB4 binding than NMO.
- In vitro culture, phagocytosis, and gamma-IFN treatment enhanced GSIB4 binding in NMO.
- A specific sequence of stimuli (gamma-IFN followed by phagocytosis) maximized GSIB4 binding.
- Metabolic processing of signals over time (1-24 hr) was required for enhanced GSIB4 binding.
Conclusions:
- Macrophage binding of GSIB4 is differentially regulated by various stimuli.
- Soluble mediators and internalization events can increase the percentage of GSIB4-positive macrophages.
- Enhanced GSIB4 binding serves as a valuable marker for assessing macrophage activation and responsiveness to stimuli.