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Updated: Apr 27, 2026

Expression and Purification of the Human Lipid-sensitive Cation Channel TRPC3 for Structural Determination by Single-particle Cryo-electron Microscopy
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Physiological functions and regulation of TRPC channels

Hwei Ling Ong1, Lorena B de Souza, Kwong Tai Cheng

  • 1Secretory Physiology Section, Molecular Physiology and Therapeutics Branch, National Institute of Dental and Craniofacial Research, National Institutes of Health, Bethesda, MD, 20892, USA.

Handbook of Experimental Pharmacology
|June 26, 2014
PubMed

Insights

TRP-canonical (TRPC) channels, crucial for cell signaling, are regulated by PIP2 hydrolysis and interact with STIM1/Orai1. TRPC6 channels are linked to human diseases, highlighting their physiological importance.

Area of Science:

  • Molecular Biology
  • Cell Physiology
  • Ion Channel Function

Background:

  • The TRP-canonical (TRPC) subfamily comprises seven Ca(2+)-permeable cation channels.
  • TRPC channels are activated by receptor-mediated PIP2 hydrolysis through store-dependent and independent pathways.
  • TRPC6 is implicated in human channelopathies, underscoring its clinical relevance.

Purpose of the Study:

  • To elucidate the regulatory mechanisms of TRPC channel activity.
  • To understand the role of STIM1 and Orai1 in TRPC channel gating.
  • To investigate the physiological significance of Ca(2+) influx mediated by TRPC channels.

Main Methods:

  • Investigating TRPC channel activation mechanisms.
  • Analyzing the interaction between TRPC channels, STIM1, and Orai1.
  • Studying the localization and regulation of TRPC channels in plasma membrane microdomains.

Main Results:

  • TRPC channels are regulated by PIP2 hydrolysis and interact with STIM1/Orai1 for gating.
  • Ca(2+) influx through TRPC channels generates specific intracellular signals.
  • Scaffolding, trafficking, and regulatory proteins coordinate TRPC channel function.

Conclusions:

  • TRPC channels play vital roles in diverse physiological processes.
  • Precise regulation of TRPC channel activity is essential for cell function.
  • Dysregulation of TRPC channels, like TRPC6, can lead to human diseases.

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