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Exhaled HPV infection in lung cancer: role of MA at 3p

Giovanna E Carpagnano1, Donato Lacedonia1, Elisabetta Crisetti1

  • 1Department of Medical and Surgical Sciences, Institute of Respiratory Disease, University of Foggia, Foggia, Italy.

Archives of Medical Research
|June 26, 2014
PubMed
Abstract

Insights

Microsatellite alterations (MAs) at 3p are present in the exhaled breath condensate of non-small cell lung cancer (NSCLC) patients positive for human papilloma virus (HPV). These MAs may play a role in lung carcinogenesis and could be useful for non-invasive screening.

Area of Science:

  • Oncology
  • Genetics
  • Virology

Background:

  • Human papilloma virus (HPV) infection is increasingly linked to lung cancer development.
  • HPV DNA integration into the host genome is a critical event in oncogenesis.
  • Fragile sites like FRA3B, near FHIT, are prone to microsatellite alterations (MAs) upon HPV integration.

Purpose of the Study:

  • To investigate the role of MAs at chromosomal region 3p in non-small cell lung cancer (NSCLC) with HPV positivity.
  • To explore correlations between 3p MAs and clinical factors such as sex, histotype, TNM stage, and smoking status.

Main Methods:

  • Allelotyping analysis of microsatellite D3S1300 at 3p was performed on DNA from exhaled breath condensate (EBC), blood, and bronchial brushing.
  • 26 NSCLC patients were analyzed, categorized into HPV-positive (11) and HPV-negative (15) groups.

Main Results:

  • The study identified MAs at 3p in the EBC of HPV-positive NSCLC patients for the first time.
  • MAs detected in EBC samples corresponded with those in paired bronchial brushing samples.
  • A higher prevalence of 3p MAs was observed in HPV-positive NSCLC patients compared to HPV-negative patients.
  • No significant correlation was found between MAs and patient sex, histotype, or tumor stage.

Conclusions:

  • MAs at 3p are present in Caucasian NSCLC patients with HPV positivity and may contribute to lung carcinogenesis.
  • Analysis of 3p MAs in EBC of HPV-positive patients shows potential for non-invasive lung cancer screening.
  • Further research is warranted to validate the clinical utility of 3p MA analysis in EBC for lung cancer detection.
Keywords:
EBCHPVLung cancerMicrosatellite alterationsNoninvasive method

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