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Calbindin-D28K Prevents Staurosporin-induced Bax Cleavage and Membrane Permeabilization

Won-Seok Choi1, Young J Oh2

  • 1School of Biological Sciences and Technology, College of Natural Sciences, College of Medicine, Chonnam National University, Gwangju 500-757, Korea. ; Department of Biology, Yonsei University College of Life Science and Biotechnology, Seoul 120-749, Korea.

Experimental Neurobiology
|June 26, 2014
PubMed

Insights

Calbindin-D28K protein protects neurons from cell death by inhibiting both apoptosis and necrosis. It prevents calcium increase and calpain activation, crucial steps in these damaging pathways.

Area of Science:

  • Neuroscience
  • Cell Biology
  • Biochemistry

Background:

  • Calbindin-D28K is known to regulate neuronal cell death.
  • Previous studies showed its role in preventing apoptosis via caspase activation.
  • Its role in staurosporine-induced calpain activation and necrosis was unclear.

Purpose of the Study:

  • To investigate the role of Calbindin-D28K in staurosporine-induced calpain activation and necrotic cell death.
  • To determine if Calbindin-D28K affects intracellular calcium levels and calpain activity.

Main Methods:

  • Neuronal cell line treated with staurosporine (STS).
  • Overexpression of Calbindin-D28K and use of calcium chelator BAPTA.
  • Measurement of intracellular calcium levels.
  • Analysis of Bax cleavage by calpain.
  • Assessment of plasma membrane permeabilization and nucleolus morphology.

Main Results:

  • Staurosporine increased intracellular calcium within 1 hour.
  • Calbindin-D28K overexpression and BAPTA prevented this calcium increase.
  • Calbindin-D28K inhibited staurosporine-induced cleavage of Bax by calpain.
  • Calbindin-D28K prevented plasma membrane permeabilization and nucleolar changes induced by staurosporine.

Conclusions:

  • Calbindin-D28K protects neurons from necrotic cell death by preventing calpain activation.
  • These findings expand the known protective functions of Calbindin-D28K beyond the caspase-apoptosis pathway.
Keywords:
calbindin-D28kcalciumcalpainneuronal cell death

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