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Investigation of genotoxicity in acromegaly from peripheral blood lymphocyte cultures using a micronucleus assay
Oguz Kaan Unal1, Nilufer Cinkilic, Ozen Oz Gul
1Department of Endocrinology and Metabolism (O.K.U., O.O.G., S.C., C.E., E.T.), Uludag University Medical School, and Department of Biology (N.C., O.V., D.Y.), Uludag University Science and Arts Faculty, 16059 Bursa, Turkey; Department of Endocrinology and Metabolism (O.K.U.), Acibadem University Medical School, 16110 Bursa, Turkey; State Hospital (O.O.G.), 16090 Bursa, Turkey; and Sevket Yilmaz Research Hospital (S.C.), 16310 Bursa, Turkey.
Context:
Although patients with acromegaly may have an increased risk of developing several types of cancers, the degree of risk for malignancy in these patients is unresolved.
Objective:
The present study aimed to investigate the potential genotoxic effects of acromegaly on the cell cycle in peripheral blood lymphocyte cultures.
Design:
This was a single center, crossover, case-control study conducted on the acromegalic patients in Turkey.
Setting:
The study was conducted in the outpatient clinic of a university hospital.
Patients:
Seventy-one consecutively screened acromegalic patients and 56 controls participated in the study.
Intervention:
Patients were included, regardless of the disease activity status and their treatment duration before the study.
Main Outcome Measures:
The primary end point was the frequency of micronucleus (MN) in the peripheral blood lymphocyte cultures, and the secondary end point was its clinical correlations.
Results:
The MN level was 3.82 ± 1.49 in the control group and 18.00 ± 6.13 in the acromegalic group (P < .01), whereas the nuclear division index (NDI) was 1.79 ± 0.12 in the control group and 1.68 ± 0.07 in the acromegalic group (P < .01). Neither MN nor NDI was correlated with age, GH, IGF-I, initial GH, initial IGF-I, duration of the remission period, and initial tumor size. Only the MN level was positively correlated with the duration of disease (r = 0.323, P = .014).
Conclusion:
Our results indicated that acromegalic patients had genotoxic damage at a substantial level, and there was a positive correlation between the duration of disease and genotoxicity level.
