[Proteomic differential display analysis of high glucose load human U937 cell line stimulated by vitamin D3]

The purpose of this study was to analysis on the affection of key protein expression by vitamin D3, and to investigate the mechanism of vitamin D3 on contributing to diabetes.

Good status U937 cells were cultured by high glucose medium which contained 45 mmol/L D-Glucose, and treated by 1, 25-(OH)(2)-D3. The control groups were U937 cells cultured by high glucose. The whole cell proteins were extracted and separated by 2-dimentional gel electrophoresis (2-DE), differential expression proteins were identified by matrix-assisted laser desorption/ionization-time of flight-mass spectrometry (MALDI-TOF-MS).

There were 30 protein spots whose expression was significantly different between vitamin D-treated and untreated high glucose loaded cells. Six protein spots were identified by MALDI-TOF-MS, three of which, prohibitin, heat shock 70 kD protein 8, and catalase were protection of cell stress proteins, one of which, electron transport flavoprotein, was respiratory chain related proteins, two of which, fumarylacetoacetate hydrolase and triosephosphate isomerase, were cell metabolism related proteins.

The differentially expressed proteins of high glucose load human U937 cell line stimulated by vitamin D3 were found. These differential proteins were mainly related to oxidative stress and energy metabolism. These data suggested that 1, 25-(OH)(2)-D3 might regulate cell high glucose load by reduction oxidative stress injury and affection energy metabolism.