Estrogenic activity of organic extracts in the effluents treated byivity present treatment and the new process

Evaluate the estrogenic activity of organic extracts in the effluents treated by present treatment and the new technique find the scientific evidences of the new wastewater treatment technique to compare the removal efficiency of trace organic pollutants.

The solid phase extraction was adopted to enrich the trace organic pollutants in the water samples with resin adsorbing, then detected the estrogenic activity of them by using yeast estrogens screen and immature rat uterine bioassay.

Yeast estrogens screen demonstrated that the organic extracts in the new technique effluent showed the activity as estrogen after concentrated 1000 times, because the activity of beta-galactosidase produced by yeast began to appear, otherwise, the same phenomenon occurred for the tertiary effluent, the secondary effluent and the influent at concentration of 500 times. At same concentration times of the extracts, the activity of beta-galactosidase of each group could be listed as, the new technique effluent < the tertiarity effluent < the secondary effluent < the influent. The immature rat uterine bioassay showed there was significant difference only between the high dose group of the influent organic extracts and the negative control (P < 0.05), but not between other groups, about the ratio of uterine weight to body weight.

The estrogenic activity of urban sewage in Zhengzhou was significantly decreased after treated but it still possesses potential hazard to environment. And the trace organic pollutants in wastewater were removed by the new technique more efficiently than by the present treatment.