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Related Concept Videos

Mesenchymal Stem Cells01:19

Mesenchymal Stem Cells

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Mesenchymal stem cells (MSCs) are adult stem cells that can differentiate into most connective tissue cell types, except for hematopoietic cells, depending upon the source of MSCs. For example, bone-marrow-derived MSCs (BM-MSCs) can differentiate into osteocytes, hepatocytes, and pancreatic and neuronal cells. MSCs can be isolated from various sources such as bone marrow, placenta, adipose tissue, teeth, and Wharton’s jelly, a gelatinous substance in the umbilical cord. The ease of their...
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Two Methods for Establishing Primary Human Endometrial Stromal Cells from Hysterectomy Specimens
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Mesenchymal stem/stromal cells in post-menopausal endometrium.

D Ulrich1, K S Tan2, J Deane2

  • 1The Ritchie Centre, MIMR-PHI Institute of Medical Research, 27-31 Wright Street, Clayton, VIC 3168, Australia Monash University Department of Obstetrics and Gynaecology, Monash Medical Centre, 246 Clayton Road, Clayton, VIC 3168, Australia Present address: Department of Obstetrics and Gynaecology, Medical University Graz, 8045 Graz, Austria.

Human Reproduction (Oxford, England)
|June 27, 2014
PubMed
Summary

Post-menopausal endometrium contains mesenchymal stem/stromal cells (MSC) with stem cell properties. These cells can be isolated via biopsy, regardless of estrogen therapy, offering a potential source for cell-based therapies.

Keywords:
W5C5endometriummesenchymal stem cellsmesenchymal stromal cellspost-menopausal

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Area of Science:

  • Stem Cell Biology
  • Regenerative Medicine
  • Gynecology

Background:

  • Mesenchymal stem/stromal cells (MSCs) have been identified in premenopausal endometrium.
  • These premenopausal endometrial MSCs can be prospectively isolated using the W5C5/SUSD2 marker.

Purpose of the Study:

  • To determine if post-menopausal endometrium contains mesenchymal stem/stromal cells (MSCs).
  • To investigate if these post-menopausal endometrial MSCs possess adult stem cell properties.
  • To assess the feasibility of prospectively isolating these cells from endometrial biopsies.

Main Methods:

  • Collected endometrial tissue from premenopausal and post-menopausal women (with or without estrogen therapy).
  • Isolated and purified W5C5(+) cells from endometrial tissue.
  • Assessed MSC properties including clonogenicity, multipotency (adipogenic, chondrogenic, osteogenic, myogenic differentiation), and surface phenotype via flow cytometry.

Main Results:

  • A population of stromal cells with MSC properties was successfully purified from post-menopausal endometrium.
  • These cells exhibited clonogenicity, multipotency, and characteristic MSC surface markers, irrespective of estrogen treatment.
  • Post-menopausal endometrial MSCs demonstrated comparable properties to premenopausal endometrial MSCs.

Conclusions:

  • Post-menopausal endometrium is a viable source of mesenchymal stem/stromal cells with key stem cell characteristics.
  • These cells can be isolated via simple endometrial biopsy, offering potential for autologous cell-based therapies.
  • Endometrial MSCs in post-menopausal women represent a promising cell source independent of age.