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MALDI-TOF Mass Spectrometry01:19

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Mass spectrometry is a powerful characterization technique that can identify and separate a wide variety of compounds ranging from chemical to biological entities, based on their mass-to-charge ratio (m/z). The instruments that allow this detection, known as mass spectrometers, have three components: an ion source, a mass analyzer, and a detector. These spectrometers differ based on the nature of their ion source and analyzers.Matrix-assisted laser desorption ionization (MALDI) is a commonly...
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Mass spectrometry is an analytical technique used to determine the molecular mass and molecular formula of a compound. The basic principle of mass spectrometry is to generate ions from the analyte molecule and measure these ion abundances against their molecular mass. One common type of ionization, known as electron ionization or EI, bombards the analyte molecules in the gas phase with high-energy electron beams. The electron beams displace an electron from the molecule and leave behind a...
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Tandem mass spectrometry is a technique that uses multiple mass analyzers in series to obtain a higher selectivity and reduce chemical noise during analyte detection. Instruments with multiple analyzers separated by an interaction cell enable secondary fragmentation and selected study of the fragment ions.Secondary fragmentations occur in the interaction cell and can be induced by various factors. Fragmentation induced by collision with inert gases, such as N2, Ar, He, etc., is called...
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This lesson details the instrumentation of a mass spectrometer—a physical instrument to perform mass spectrometry on analyte molecules and record the characteristic mass spectra. This is achieved via three chief functions:
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Peptide Identification Using Tandem Mass Spectrometry01:33

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Tandem mass spectrometry, also known as MS/MS or MS2, is an analytical technique that employs two mass analyzers. Essentially it is a series of mass spectrometers that helps isolate a particular biomolecule and then helps study its chemical properties.
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Mass++: A Visualization and Analysis Tool for Mass Spectrometry.

Satoshi Tanaka1, Yuichiro Fujita1, Howell E Parry1

  • 1Koichi Tanaka Laboratory of Advanced Science and Technology, Shimadzu Corporation , Kyoto 604-8511, Japan.

Journal of Proteome Research
|June 27, 2014
PubMed
Summary
This summary is machine-generated.

Mass++ is a customizable mass spectrometry tool for proteomics and metabolomics. This software development platform allows rapid analysis and custom function creation for mass spectrometry (MS) data.

Keywords:
Mass++MassBankidentificationmass spectrometryplatformplug-inquantitation

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Area of Science:

  • Biochemistry
  • Computational Biology
  • Analytical Chemistry

Background:

  • Mass spectrometry (MS) is crucial for analyzing biomolecules.
  • Existing MS analysis tools often lack flexibility and customizability.
  • There is a need for adaptable platforms for advanced proteomics and metabolomics research.

Purpose of the Study:

  • To introduce Mass++, a novel plug-in style software for mass spectrometry data analysis.
  • To highlight the extensibility and customizability of Mass++ through its plug-in architecture.
  • To demonstrate the utility of Mass++ for rapid MS data analysis and custom function development.

Main Methods:

  • Development of Mass++ as a modular, plug-in based software.
  • Implementation of various plug-ins for data visualization and analysis in proteomics and metabolomics.
  • Support for multiple vendor-specific raw data formats.
  • Demonstration of custom function development using label-free quantitation as an example.

Main Results:

  • Mass++ provides a flexible platform for mass spectrometry data analysis.
  • The plug-in architecture allows for easy customization and addition of new functionalities.
  • The tool supports diverse data formats and analysis methods, including proteomics and metabolomics.
  • Rapid analysis and function development capabilities were demonstrated.

Conclusions:

  • Mass++ offers a versatile and extensible solution for mass spectrometry data processing.
  • Its plug-in style architecture empowers users to tailor the tool for specific research needs.
  • Mass++ serves as both a powerful analysis tool and a software development platform for MS researchers.