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Development of multiplex loop-mediated isothermal amplification assays to detect medically important yeasts in dairy

Kohei Kasahara1, Hiroshi Ishikawa, Sumie Sato

  • 1Yakult Central Institute for Microbiological Research, Kunitachi, Tokyo, Japan.

FEMS Microbiology Letters
|June 27, 2014
PubMed

Insights

Rapid loop-mediated isothermal amplification (LAMP) assays detect common pathogenic yeasts like Candida and Trichosporon in food. These quick, cost-effective methods offer sensitive yeast contamination screening within an hour.

Area of Science:

  • Microbiology
  • Food Science
  • Molecular Biology

Background:

  • Yeast contamination poses a significant risk in the food industry.
  • Emerging opportunistic pathogenic yeasts such as Candida and Trichosporon species can cause serious infections.

Purpose of the Study:

  • To develop rapid and sensitive loop-mediated isothermal amplification (LAMP) assays for detecting specific pathogenic yeasts.
  • To create multiplex LAMP assays for simultaneous detection of multiple yeast species in food products.

Main Methods:

  • Designed species-specific LAMP primer sets targeting the ITS2 region for Candida and IGS1 region for Trichosporon.
  • Validated LAMP assays for sensitivity, detecting yeasts from 10^0 to 10^3 cells/mL in contaminated dairy products within 1 hour.
  • Developed and tested multiplex LAMP assays for simultaneous detection of target Candida or Trichosporon species.

Main Results:

  • LAMP assays demonstrated high sensitivity and rapid detection capabilities for target yeasts.
  • Multiplex LAMP assays effectively detected the presence of at least one target species.
  • The developed assays were time- and cost-efficient compared to conventional methods.

Conclusions:

  • Loop-mediated isothermal amplification (LAMP) assays provide a rapid and reliable method for detecting pathogenic yeast contamination in food.
  • Multiplex LAMP assays serve as an efficient primary screening tool for yeast contamination in food products.
  • These assays contribute to enhanced food safety by enabling quick identification of potential health hazards.
Keywords:
CandidaTrichosporonfoodloop-mediated isothermal amplificationmultiplexyeast

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