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Related Experiment Videos

Mouse carbonic anhydrase III: nucleotide sequence and expression studies.

S Tweedie1, Y Edwards

  • 1MRC Human Biochemical Genetics Unit, The Galton Laboratory, University College London, U.K.

Biochemical Genetics
|February 1, 1989
PubMed
Summary

Researchers isolated mouse carbonic anhydrase III (CAIII) cDNA, finding high homology with human CAIII. Mouse CAIII is abundant in skeletal muscle and liver, with no observed sex-related distribution differences, unlike in rats.

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Area of Science:

  • Molecular Biology
  • Biochemistry
  • Genetics

Background:

  • Carbonic anhydrase III (CAIII) is an enzyme implicated in various physiological processes.
  • Understanding the genetic and expression patterns of CAIII is crucial for its functional characterization.

Purpose of the Study:

  • To isolate and characterize the cDNA for mouse carbonic anhydrase III (CAIII).
  • To compare the mouse CAIII sequence with its human counterpart.
  • To investigate the expression patterns and distribution of CAIII in mouse tissues.

Main Methods:

  • Isolation of mouse CAIII cDNA using a lambda gt11 expression library.
  • Nucleotide and amino acid sequence homology analysis with human CAIII.
  • Protein and mRNA expression analysis in cultured myoblasts, skeletal muscle, and liver.

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  • Restriction fragment length polymorphism (RFLP) analysis for genetic differentiation.

    • Main Results:

    • The cloned mouse CAIII cDNA contains the complete coding region and untranslated sequences.
    • Mouse CAIII exhibits high nucleotide (87%) and amino acid (91%) homology to human CAIII.
    • CAIII is expressed at low levels in myoblasts and abundantly in adult skeletal muscle and liver.
    • Unlike rats, no sex-related differences in CAIII distribution were observed in mice.
    • TaqI and PstI restriction fragment length polymorphisms differentiate between Mus spretus and Mus musculus domesticus.

    Conclusions:

    • The successful isolation and characterization of mouse CAIII provide a valuable resource for further research.
    • The high homology suggests conserved function between mouse and human CAIII.
    • Tissue-specific expression patterns and the absence of sex-related differences in mice offer insights into CAIII regulation and function.