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Updated: Jul 15, 2026

Simultaneous pH Measurement in Endocytic and Cytosolic Compartments in Living Cells using Confocal Microscopy
Published on: April 28, 2014
A novel nanogel-based fluorescent probe for ratiometric detection of intracellular pH values
Lixia Cao1, Xiaoyan Li, Shuangqing Wang
1Beijing National Laboratory for Molecular Sciences, Key Laboratory of Photochemistry, Institute of Chemistry, Chinese Academy of Sciences, Beijing 100190, China. gqyang@iccas.ac.cn shayuli@iccas.ac.cn.
Abstract:
A new nanogel indicator (NGI) for ratiometric fluorescent detection of intracellular pH values has been reported. In aqueous solution, the NGI can be used for ratiometric sensing of pH changes with a large hypsochromic shift of 100 nm in the green-red region and reversible. Furthermore, the NGI has been used to calibrate the cytosol pH value in living cells.

