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Updated: Apr 27, 2026

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Stable DNA Motifs, 1D and 2D Nanostructures Constructed from Small Circular DNA Molecules
Published on: April 12, 2019
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Self-assembled DNA nanostructures prepared by rolling circle amplification for the delivery of siRNA conjugates
Cheol Am Hong1, Bora Jang, Eun Hye Jeong
1College of Pharmacy, Graduate School of Pharmaceutical Sciences, Ewha Womans University, Seoul 120-750, Republic of Korea. hyukjin@ewha.ac.kr.
Abstract:
Inspired by the isothermal enzymatic process of rolling circle amplification (RCA) of DNA strands, we have developed a system to achieve more than a 200-fold increase in the synthesis of DNA nanostructures using a single-stranded circular DNA template. The amplified DNA nanostructures have shown efficient delivery of folic acid (FA) conjugated siRNAs into KB cells with a dose dependent gene silencing.

