Related Experiment Video

Updated: Apr 27, 2026

Multiplex PCR Assay for Typing of Staphylococcal Cassette Chromosome Mec Types I to V in Methicillin-resistant Staphylococcus aureus
09:03

Multiplex PCR Assay for Typing of Staphylococcal Cassette Chromosome Mec Types I to V in Methicillin-resistant Staphylococcus aureus

Published on: September 5, 2013

11.4K

[Mobile genetic elements associated with erythromycin-resistant isolates of Streptococcus pneumoniae in Colombia]

Viviana Ramos1, Carolina Duarte2, Aleyda Díaz3

  • 1Grupo de Microbiología, Subdirección de Investigación Científica y Tecnológica, Instituto Nacional de Salud.

Biomedica : Revista Del Instituto Nacional De Salud
|June 27, 2014
PubMed
Abstract

Insights

Macrolide-resistant Streptococcus pneumoniae in Colombia are increasingly linked to mobile genetic elements like transposons. These elements carry multiple antibiotic resistance genes, contributing to growing antimicrobial resistance challenges.

Area of Science:

  • Microbiology
  • Genetics
  • Epidemiology

Context:

  • Increasing macrolide antibiotic resistance in Streptococcus pneumoniae clinical isolates.
  • Association between resistance and mobile genetic elements.

Purpose:

  • Determine serotype and transposon distribution in macrolide-resistant invasive S. pneumoniae isolates in Colombia (1994-2011).

Summary:

  • Analyzed 225 macrolide-resistant S. pneumoniae isolates.
  • Identified cMLS B (62.7%), iMLS B (2.7%), and M (34.6%) phenotypes.
  • Prevalent transposons included Tn 5253 (34%), Tn 1545 (18.4%), and Tn 3872 (14.9%), often linked to specific serotypes and international clones. Mega element (62.8%) associated with M phenotype.

Impact:

  • The majority of erythromycin-resistant S. pneumoniae isolates exhibited the cMLS B phenotype, associated with transposons carrying multiple resistance determinants.
  • Isolates with M phenotype carried the mef(E) gene within the mega element, highlighting the role of mobile genetic elements in multidrug resistance.

More Related Videos

Constructing Mutants in Serotype 1 Streptococcus pneumoniae strain 519/4306:06

Constructing Mutants in Serotype 1 Streptococcus pneumoniae strain 519/43

Published on: September 11, 2020

1.8K
Detection of Horizontal Gene Transfer Mediated by Natural Conjugative Plasmids in E. coli06:56

Detection of Horizontal Gene Transfer Mediated by Natural Conjugative Plasmids in E. coli

Published on: March 24, 2023

9.4K

Related Concept Videos

Mechanism of Antibiotic Resistance in MRSA01:25

Mechanism of Antibiotic Resistance in MRSA

Antibiotic resistance in bacteria arises when microorganisms evolve the ability to withstand drugs designed to kill them or inhibit their growth, rendering once-effective treatments useless. This phenomenon, driven by genetic change and selection under antibiotic exposure, poses a profound threat to modern medicine. Mechanisms include drug-inactivating enzymes (e.g., β-lactamases), efflux pumps that eject antibiotics, mutations altering antibiotic targets, decreased drug uptake, and...
211
Clinical Significance of Antibiotic Resistance01:25

Clinical Significance of Antibiotic Resistance

Methicillin-resistant Staphylococcus aureus (MRSA) presents a critical public health threat, arising from its capacity to resist β-lactam antibiotics due to acquisition of the mecA gene within the staphylococcal cassette chromosome mec (SCCmec). This gene encodes penicillin-binding protein 2a (PBP2a), which impairs binding efficacy of methicillin and other β-lactams. MRSA has evolved into distinct clonal lineages impacting humans and animals alike, reinforcing its significance within...
85
Atypical Pneumonia01:14

Atypical Pneumonia

Atypical pneumonia, often caused by Mycoplasma pneumoniae, is a form of pulmonary infection that differs from the classical presentation of bacterial pneumonia in both its cause and clinical symptoms. Mycoplasma pneumoniae is a pleomorphic bacterium notable for its lack of a rigid cell wall. This structural characteristic imparts resistance to beta-lactam antibiotics and significantly influences the bacterium’s behavior within the human host.Other pathogens responsible for the disease...
80
Development of Antibiotic Resistance01:30

Development of Antibiotic Resistance

Antibiotic resistance is a major public health concern that arises when bacteria evolve mechanisms to withstand the effects of antibiotic treatments. This resistance can be intrinsic, acquired through genetic mutations, or transferred between bacteria via horizontal gene transfer. The development of antibiotic resistance poses significant challenges in treating bacterial infections and necessitates ongoing research to develop new therapeutic strategies.Intrinsic resistance occurs when bacterial...
2.0K