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Updated: Apr 27, 2026
Detection of Functional Matrix Metalloproteinases by Zymography
Published on: November 8, 2010
[Quantitative detection of matrix metalloproteinase-2 in normal coronal dentine of young people]
Danyang Wang1, Ling Zhang2, Fang Li
1Department of Prosthodontics, College of Stomatology, The Fourth Military Medical University, Xi'an 710032, China.
Objective:
To compare the distribution and concentration of matrix metalloproteinases-2 (MMP-2) in different dentin depth of premolar and molar of young people.
Methods:
Freshly extracted human premolars and molars (aged between 20-30) were sectioned to 1.5 mm thick slices along the longitudinal axis of the tooth separately. Enamel and pulp of each slice was removed, and then the premolar and molar slices were respectively divided into two subgroups according to superficial or deep dentin and pulverized to fine powder. After dentin protein was extracted, the concentrations of MMP-2 in different tooth were detected using fluorescent microsphere immunoassay.
Results:
The content of MMP-2 in superficial layer dentin of premolar was (0.022 ± 0.006) ng/mg. The content of MMP-2 in deep layer dentin of premolar was (2.087 ± 0.090) ng/mg. The content of MMP-2 in superficial layer dentin of molar was (0.336 ± 0.037) ng/mg. The content of MMP-2 in deep layer dentin of molar was (3.312 ± 0.308) ng/mg.
Conclusions:
MMP-2 exists in human coronal dentin. In the same type of teeth of young people, the concentration of MMP-2 in deep dentin was significant higher than those in superficial dentin. In the same dentin depth, the concentration of MMP-2 in molar was significant higher than those in premolar.
