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Wogonoside induces autophagy-related apoptosis in human glioblastoma cells

Li Zhang1, Handong Wang1, Zixiang Cong1

  • 1Department of Neurosurgery, Jinling Hospital, School of Medicine, Nanjing University, Nanjing, Jiangsu 210002, P.R. China.

Oncology Reports
|June 28, 2014
PubMed

Insights

Wogonoside, a flavonoid from Scutellaria baicalensis, effectively kills glioblastoma cells by inducing apoptosis and autophagy. Autophagy blockade reduces its anticancer effect, suggesting wogonoside

Area of Science:

  • Pharmacology
  • Molecular Biology
  • Oncology

Background:

  • Wogonoside is a flavonoid from Scutellaria baicalensis with preclinical anticancer activity.
  • Its effects on glioblastoma, a type of malignant glioma, are not well understood.

Purpose of the Study:

  • To investigate the effects of wogonoside on human glioblastoma cells.
  • To elucidate the mechanisms underlying wogonoside's potential anticancer activity in glioblastoma.

Main Methods:

  • Cell viability assays were performed on glioblastoma cells treated with wogonoside.
  • Mitochondrial apoptosis, autophagy markers (LC3 dots, AVO formation, p62 degradation), and signaling pathways (MAPK, PI3K/AKT/mTOR) were analyzed.
  • Autophagy was inhibited using chemical inhibitors and RNA interference to assess its role.

Main Results:

  • Wogonoside demonstrated significant cytotoxic effects on human glioblastoma cells.
  • Cell death was primarily mediated by the induction of mitochondrial apoptosis.
  • Wogonoside treatment promoted autophagy, evidenced by increased LC3 puncta, AVO formation, and p62/LC3 turnover.
  • Inhibition of autophagy diminished the anticancer efficacy of wogonoside.
  • The p38 MAPK and PI3K/AKT/mTOR pathways, along with ROS, were implicated in wogonoside-induced apoptosis and autophagy.

Conclusions:

  • Wogonoside exhibits anticancer properties against human glioblastoma cells.
  • The compound induces apoptosis and enhances autophagy, with autophagy playing a crucial role in its efficacy.
  • Wogonoside-induced autophagy and apoptosis involve the p38 MAPK, PI3K/AKT/mTOR pathways, and ROS.
  • Wogonoside warrants further investigation as a potential therapeutic agent for malignant glioma.

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